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目的:探讨miR-203在肝细胞肝癌(Hepatocellular carcinoma,HCC)患者血浆中的表达及其对肝癌细胞增殖和侵袭能力的影响。方法:选择2013年5月-2015年7月在我院确诊为肝细胞肝癌的患者57例作为研究对象,另选取同期在我院接受健康体检的志愿者49例作为对照组。采用实时荧光定量RT-PCR法检测两组血浆中miR-203的表达水平;将过表达的miR-203转染至人肝癌细胞系Hep 3B和JHH-1;采用CCK-8法检测miR-203对肝癌细胞增殖能力的影响;采用transwell法检测miR-203对肝癌细胞迁移及侵袭能力的影响。结果:miR-203在肝癌患者血浆中的表达水平显著低于对照组,差异具有统计学意义(P<0.01);与未转染的肝癌细胞比较,Hep 3B和JHH-1细胞转染miR-203mimic后,肿瘤细胞的增殖、迁移及侵袭能力受到明显抑制,差异具有统计学意义(P<0.05)。结论:miR-203在肝细胞肝癌中呈低表达,而过表达miR-203能够抑制肿瘤细胞的增殖及侵袭能力,提示miR-203可以作为一种新的肿瘤抑制性micro RNA。
Objective: To investigate the expression of miR-203 in plasma of patients with hepatocellular carcinoma (HCC) and its effect on the proliferation and invasion of hepatocellular carcinoma cells. Methods: 57 cases of hepatocellular carcinoma diagnosed in our hospital from May 2013 to July 2015 were selected as the research object, and 49 volunteers who were in our hospital during the same period were selected as the control group. The expression of miR-203 in plasma was detected by real-time fluorescent quantitative RT-PCR. The overexpressed miR-203 was transfected into human hepatocellular carcinoma cell lines Hep 3B and JHH-1. The expression of miR-203 On the proliferation of hepatocellular carcinoma cells. The transwell assay was used to detect the effect of miR-203 on the migration and invasion of hepatocellular carcinoma cells. Results: The expression of miR-203 in the plasma of patients with hepatocellular carcinoma was significantly lower than that of the control group (P <0.01). Compared with untransfected hepatocellular carcinoma cells, Hep 3B and JHH-1 cells transfected with miR- 203mimic, the proliferation, migration and invasiveness of tumor cells were significantly inhibited, the difference was statistically significant (P <0.05). Conclusion: miR-203 is low expressed in hepatocellular carcinoma, while overexpression of miR-203 can inhibit the proliferation and invasion of tumor cells, suggesting that miR-203 may serve as a novel tumor suppressor microRNA.