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目的：观察高三尖杉酯碱（ｈｏｍｏｈａｒｒｉｎｇｔｏｎｉｎｅ，ＨＨＴ）对视网膜母细胞瘤（ｒｅｔｉｎｏｂｌａｓｔｏｍａ，ＲＢ）细胞系ＨＸＯ－ＲＢ４４细胞的杀伤作用及其诱导的细胞死亡形式。方法：用ＭＴＴ法测定肿瘤细胞存活率，提取药物作用后的细胞核ＤＮＡ进行琼脂糖凝胶电泳。结果：ＨＨＴ在１０－９ｍｏｌ／Ｌ～１０－４ｍｏｌ／Ｌ浓度范围对ＨＸＯ－ＲＢ４４具有较强的杀伤作用（Ｐ＜０．０５）。１０－６ｍｏｌ／Ｌ的ＨＨＴ与ＨＸＯ－ＲＢ４４作用４８小时后，核ＤＮＡ发生有规律的裂解，琼脂糖凝胶电泳呈典型ＤＮＡ梯度。结论：ＨＨＴ诱导ＲＢ发生程序性死亡（ｐｒｏｇｒａｍｍｅｄｃｅｌｄｅａｔｈ，ＰＣＤ），其诱导活性呈较强的浓度和时间依赖性。
OBJECTIVE: To observe the killing effect of homoharringtonine (HHT) on retinoblastoma (RB) cell line HXO-RB44 and its induced cell death pattern. Methods: The survival rate of tumor cells was determined by MTT method. The DNA of the cells after the drug was extracted was subjected to agarose gel electrophoresis. Results: HHT could kill HXO-RB44 in a concentration range of 10-9mol / L ~ 10-4mol / L (P <0.05). Forty-eight hours after treatment with 10-6mol / L HHT and HXO-RB44, the nuclear DNA was cleaved regularly, and the DNA ladder showed a typical DNA gradient on agarose gel electrophoresis. Conclusion: HHT induces programmed programmed death (PCD) and its inducing activity is in a concentration-and time-dependent manner.