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本文对硬毛粗盖孔菌子实体不同提取物的抗肿瘤活性进行筛选。通过建立H_(22)荷瘤小鼠肿瘤移植模型,研究硬毛粗盖孔菌子实体的石油醚提取物、二氯甲烷提取物、乙酸乙酯提取物、甲醇提取物以及水提取物等5种提取物的高、中、低剂量组对H_(22)荷瘤小鼠的抑瘤作用,并对其H_(22)荷瘤小鼠的抑瘤率、体质量、胸腺指数、脾脏指数、肝脏指数、肾脏指数进行了考察。对小鼠肿瘤、脾脏、肾脏进行了组织病理学检查,对血清中的细胞因子IL‐2、IL‐4、IFN‐γ及TNF‐α的含量进行测定。结果表明:各组分均有抑瘤效果,其中乙酸乙酯提取物中剂量组(1 000mg/kg)抑瘤效果最佳,与阴性对照组比较有极显著差异(P<0.01),并且这组的H_(22)荷瘤小鼠血清中白介素‐2的含量显著增加,同时肿瘤细胞通过HE染色后,可观察到出现坏死面积增大,与抑制肿瘤活性具有相关性。
In this paper, the anti-tumor activity of different extracts of the crude fruiting body Mycelium was screened. Through the establishment of H22 tumor-bearing mouse model of tumor transplantation, five kinds of petroleum ether extract, dichloromethane extract, ethyl acetate extract, methanol extract and water extract The antitumor activity of extracts of high, medium and low dose groups on H_ (22) tumor-bearing mice, and its tumor inhibition rate, body weight, thymus index, spleen index, liver Index, kidney index were investigated. The tumor, spleen and kidney of mice were examined by histopathology, and the contents of IL-2, IL-4, IFN-γ and TNF-α in serum were measured. The results showed that all the components had anti-tumor effect, among which the medium-dose ethyl acetate extract (1 000 mg / kg) had the best anti-tumor effect, which was significantly different from the negative control group (P <0.01) In the group of H22 tumor-bearing mice, the level of interleukin-2 was significantly increased. At the same time, the tumor necrosis area was observed after the tumor cells were stained by HE, which was correlated with the inhibition of tumor activity.