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目的 观察雪旺细胞 (Schwann cells,SC)源营养神经活性物质 (SC derived neurotrophic ac-tivity,SCNA)对 Sprague- Dawly(SD)大鼠视神经损伤后视网膜节细胞 (retinal ganglion cells,RGC)存活的影响。 方法 体外培养日龄 3～ 5 d SD乳鼠 SC,收集无血清条件培养液 ,经超滤浓缩后制成冻干粉。SD大鼠分为正常对照组 ,视神经夹伤对照组 ,视神经夹伤溶剂对照组 ,视神经夹伤 SCNA治疗组 ,每组 2 0只眼。荧光金逆行标记 RGC后 7d,除正常对照组外均行球后视神经夹伤 ,SCNA治疗组将 10 0 ng SCNA注入大鼠玻璃体腔内。分别于视神经夹伤后第 5、7、14、2 1、2 8d将动物灌注固定 ,做全视网膜铺片 ,行 RGC计数。 结果 视神经夹伤后第 7d RGC开始减少 ,14d时降至正常对照的 70 .2 % ,2 8d时降至 40 .5 %。 SCNA治疗组 7d时 RGC数开始减少 ,但 14、2 1、2 8d RGC数均明显多于视神经夹伤对照组及视神经夹伤溶剂对照组 (P<0 .0 1)。 结论 在视神经夹伤后眼内注射 SCNA能减少 RGC的死亡对 RGC损伤有保护作用。
Objective To observe the effect of SC derived neurotrophic ac-tivity (SCNA) on the survival of retinal ganglion cells (RGCs) after optic nerve injury in Sprague-Dawly (SD) rats influences. Methods SC neonatal SD rats aged 3 to 5 days were cultured in vitro. Serum-free conditioned medium was collected and concentrated by ultrafiltration to make lyophilized powder. SD rats were divided into normal control group, optic nerve crush control group, optic nerve crush solvent control group and optic nerve crush SCNA treatment group, 20 eyes in each group. Seven days after fluorescent gold retrograde labeling of RGC, all posterior optic nerve crush was performed except for the normal control group. SCNA treatment group was injected with 10 0 ng SCNA into the vitreous cavity of rats. Animals were perfused and fixed on the 5th, 7th, 14th, 2nd, 12th, 8th day after optic nerve injury, respectively. Results RGC started to decrease on the 7th day after optic nerve crush, decreased to 70.2% of the normal control on the 14th day and decreased to 40.5% on the 28th day. The number of RGCs in SCNA treatment group began to decrease at 7d, but the numbers of RGCs in 14,2, 12, 8d were significantly more than that in optic nerve injury control group and optic nerve crush solvent control group (P <0.01). Conclusion Intraocular injection of SCNA after optic nerve crush can reduce RGC death and protect RGC injury.