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目的研究大黄素(1,3,8-三羟基-6-甲基蒽醌)是否能减轻急性坏死性胰腺炎(ANP)大鼠肺组织的炎症反应。方法通过胰腺被膜下注射3%牛磺胆酸钠制造大鼠急性坏死性胰腺炎模型,大黄素以灌肠的方式给药,检测在急性坏死性胰腺炎给药后3、6和12h的血浆淀粉酶变化,用半定量RT-PCR方法检测大鼠肺组织IL-1β,IL-6和IL-10的表达,免疫组化方法观察肺内IL-1β转化酶(ICE,又称caspase-1)的改变。结果在未造模组血浆淀粉酶(1611.20±218.72)IU/L。在造模后3、6和12h,急性坏死性胰腺炎模型组(未用大黄素治疗组)血浆淀粉酶分别为(1981.40±56.81)IU/L,(3287.40±612.37)IU/L和(4914.60±746.82)IU/L,与未造模组比较有显著性差异(P<0.05),在给予大黄素治疗后血浆淀粉酶3、6和12h分别为(1617.20±136.80)IU/L,(2323.40±318.19)IU/L和(2670.20±390.03)IU/L。RT-PCR表明给药组与未给药组比较IL-1β和IL-6mRNA的表达随着时间的改变而降低,IL-10在mRNA上表达增加。免疫组化显示IL-1β转化酶(ICE)在各时间点给药组与未给药组比较其表达减少。结论大黄素对大鼠急性坏死性胰腺炎肺组织炎症反应有一定的抗炎作用,其抗炎作用的机制可能与大黄素下调IL-1β和IL-6mRNA的表达和上调IL-10mRNA的表达有关。
Objective To investigate whether emodin (1,3,8-trihydroxy-6-methyl hydrazine) can reduce the inflammatory response of lung tissue in rats with acute necrotizing pancreatitis (ANP). METHODS: Rat acute necrotizing pancreatitis was induced by subinjection of 3% sodium taurocholate into the pancreatic capsule. Emodin was administered enema to detect plasma starch at 3, 6 and 12 hours after administration of acute necrotizing pancreatitis. Change of enzyme, semi-quantitative RT-PCR method was used to detect the expression of IL-1β, IL-6 and IL-10 in lung tissue of rats. Immunohistochemical method was used to observe IL-1β converting enzyme in lung (ICE, also known as caspase-1). Change. The results were in the unmodeled plasma amylase (1611.20 ± 218.72) IU/L. At 3, 6 and 12 h after modeling, plasma amylase levels in the acute necrotizing pancreatitis model group (without emodin treatment group) were (1981.40±56.81) IU/L, (3287.40±612.37) IU/L and (4914.60, respectively). ± 746.82) IU/L, which was significantly different from the untreated group (P<0.05). Plasma amylase 3, 6 and 12h after administration of emodin was (1617.20±136.80) IU/L, respectively (2323.40). ± 318.19) IU/L and (2670.20 ± 390.03) IU/L. RT-PCR showed that the expression of IL-1β and IL-6 mRNA was decreased with time in the administration group and the non-administration group, and IL-10 expression was increased on the mRNA. Immunohistochemistry showed that the expression of IL-1β converting enzyme (ICE) was decreased in the administration group at each time point as compared with the non-administered group. Conclusion Emodin has certain anti-inflammatory effects on the inflammatory response of lung tissue in rats with acute necrotizing pancreatitis. Its anti-inflammatory mechanism may be related to the emodin down-regulation of IL-1β and IL-6 mRNA expression and up-regulation of IL-10 mRNA expression. .