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目的 调查上海地区汉族人群 HL A- E等位基因的分布情况及与 HL A- A、- B抗原之间的连锁不平衡关系 ,为探讨 HL A- E基因多态性与疾病的关联奠定基础。方法 HL A- A、- B抗原分型采用 NIH标准微量细胞毒方法 ;HL A- E等位基因检测采用聚合酶链反应 /序列特异的寡核苷酸探针杂交方法 ,对2 0 1名上海地区汉族正常人进行了调查。结果 HL A- E的 5个等位基因在这一人群中共检测到 3个 ,其中E* 0 10 1的频率最高 ,为 42 .2 9%,其次为 E* 0 10 32和 E* 0 10 31,分别占 32 .84%和 2 4.88%,E* 0 10 2和E* 0 10 4在所有标本中均未检出。检出的 3个等位基因与 HL A- A、- B进行两位点等位基因连锁不平衡分析 ,其观察值与理论值之间除 B15 / E* 0 10 32和 A2 / E* 0 10 32 (P<0 .0 5 )之外均无显著性差异。结论 中国上海地区汉族人群 HL A- E等位基因频率分别为 E* 0 10 1占 42 .2 9%,E* 0 10 31和 E* 0 10 32分别占2 4.88%和 32 .84%;HL A- E与 HL A- A或 - B位点等位基因之间不存在广泛的连锁不平衡。
Objective To investigate the distribution of HLA-E alleles and the linkage disequilibrium between HLA-E and HLA-B antigens in Han population of Shanghai and lay a foundation for exploring the association between HLA-E gene polymorphisms and diseases . Methods HLA - A and - B antigen typing was performed using NIH standard microcytotoxicity assay. HL - E allele was detected by polymerase chain reaction / sequence - specific oligonucleotide probe hybridization. Han normal people in Shanghai conducted a survey. Results Three alleles of HL-E were detected in this population, of which E * 0 10 1 had the highest frequency of 42.29%, followed by E * 0 10 32 and E * 0 10 31, accounting for 32.84% and 24.8%, respectively. E * 0 10 2 and E * 0 10 4 were not detected in all specimens. The three alleles detected were linked to HLA-A and-B for two-locus allele linkage disequilibrium analysis with the exception of B15 / E * 0 10 32 and A2 / E * 0 between observation and theoretical value 10 32 (P <0. 05) outside the no significant difference. Conclusion The frequencies of HLA-E alleles of Han population in Shanghai, China were 42.29% for E * 0 101, 2. 4.88% and 32.84% for E * 0 1031 and E * 0 1032, respectively. There is no extensive linkage disequilibrium between HL A- E and HL A- A or-B locus alleles.