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目的探索天龙粉经过4种蛋白酶酶解后对肺腺癌A549细胞株的抑制作用,筛选出最佳用酶。方法天龙粉分别经过胰蛋白酶、胃蛋白酶、胰凝乳蛋白酶和弹性蛋白酶酶解,采用茚三酮比色法测定蛋白质的水解度,同时以天龙粉水提物作为对照组,用甲基噻唑蓝(MTT)法测定4种酶水解物对肺腺癌A549的抑制率。结果对照组不同药物浓度对肺腺癌A549细胞无明显抑制作用;弹性蛋白酶、胰蛋白酶对天龙粉的水解度分别为77.50%、28.02%,给药组不同药物浓度对肺腺癌A549细胞的抑制率明显高于对照组;胰凝乳蛋白酶和胃蛋白酶的水解度分别为34.63%、72.80%,给药组不同药物浓度对肺腺癌A549细胞无明显抑制作用。结论天龙粉酶解工艺的最佳用酶是弹性蛋白酶,其次是胰蛋白酶。
Objective To explore the inhibitory effect of Tenlong powder on lung adenocarcinoma A549 cells after enzymatic hydrolysis by four proteases, and select the best enzyme for screening. Methods Tianlong powder was digested by trypsin, pepsin, chymotrypsin and elastase respectively. The degree of hydrolysis of protein was determined by ninhydrin colorimetry. At the same time, (MTT) assay of four kinds of enzyme hydrolyzate of lung adenocarcinoma A549 inhibition rate. Results The drug concentration of the control group had no significant inhibitory effect on lung adenocarcinoma A549 cells. The degree of hydrolysis of elastase and trypsin to the longan powder was 77.50% and 28.02%, respectively. The inhibitory effect of different drug concentrations on the lung adenocarcinoma A549 cells The rates of chymotrypsin and pepsin were 34.63% and 72.80%, respectively. The drug concentration in the drug-treated group had no obvious inhibitory effect on A549 cells. Conclusion Tianlong powder enzymatic hydrolysis process the best enzyme is elastase, followed by trypsin.