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目的:研究南湖菱壳的化学成分及体外抑制α-葡萄糖苷酶活性。方法:对醋酸乙酯和正丁醇部位采用各种色谱法分离,运用多种波谱技术鉴定结构。结果:从南湖菱的醋酸乙酯和正丁醇部位共分离鉴定9个化合物,分别为4,23,24-三甲基胆甾-22-烯-3-醇(1),豆甾醇(2),α-香树脂醇(3),(+)-nyasol(4),齐墩果酸(5),熊果酸(6),常春藤皂苷元(7),3,23-二羟基-12-烯-28-乌苏酸(8),β-胡萝卜苷(9)。南湖菱壳总提取物、石油醚部位、醋酸乙酯部位、正丁醇部位均有体外α-葡萄糖苷酶抑制活性;化合物5(IC502.88 mg·L-1)和6(IC504.42 mg·L-1)具有体外α-葡萄糖苷酶抑制活性。结论:除化合物2外,其他化合物均为首次从该属中分离得到;化合物1～9均为首次从该植物中分离得到。
Objective: To study the chemical constituents of South Lake chestnut and inhibit α-glucosidase activity in vitro. Methods: Ethyl acetate and n-butanol fractions were separated by various chromatographic methods and identified by a variety of spectroscopic techniques. Results: Nine compounds were isolated and identified from the ethyl acetate and n-butanol fractions of Nanhu Ling, namely 4,23,24-trimethylcholest-22-en-3-ol (1) (3), (+) - nyasol (4), oleanolic acid (5), ursolic acid (6), hematogen saponin (7), 3,23-dihydroxy-12 -ene-28-ursolic acid (8), beta-daucosterol (9). The inhibitory activity of α-glucosidase in total extract, petroleum ether fraction, ethyl acetate fraction and n-butanol fraction of South Lake were tested. The inhibitory activities of compound 5 (IC502.88 mg · L-1) and 6 (IC504.42 mg · L-1) has in vitro α-glucosidase inhibitory activity. CONCLUSION: All compounds except for compound 2 were isolated from this genus for the first time. Compounds 1 to 9 were isolated from this plant for the first time.