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为了分析ｐｅｒｃｏｌｌ梯度离心（ＰＧＣ）法分离小鼠附睾精子后，精子受精力提高的原因，本文测定了经洗涤和ＰＧＣ法分离后及进一步孵育后小鼠精子的胆固醇和磷脂含量，同时对附睾液和输精管液的胆固醇和磷脂含量、脂类的组成成份以及与精子共同孵育后对精子活力的影响也进行了测定。结果表明，附睾液和输精管液中存在着丰富的脂类小泡，含有高浓度的胆固醇和磷脂（０．２７３和０．７０４ｎｍｏｌｅ／μｇＤＮＡ），这些生理量的脂类与精子共同培养，能使精子失去活动力。用ＫＲＢ液洗涤后精子的胆固醇和磷脂含量分别为０．２３５和０．６９４ｎｍｏｌｅ／μｇＤＮＡ，用ＫＲＢ－ＢＳＡ洗涤和进一步孵育对精子胆固醇和磷脂含量无明显影响，二梯度ＰＧＣ法分离精子可显著降低精子的磷脂含量（０．３１５ｎｍｏｌｅ／μｇＤＮＡ）。
In order to analyze the reason why spermatozoon fertility is improved after percoll gradient centrifugation (PGC), we measured the cholesterol and phospholipid content of mouse sperm after washing and PGC separation and further incubation, And vas deferens fluid cholesterol and phospholipid content, lipid composition and co-incubation with sperm on sperm motility were also determined. The results showed that epididymal fluid and vas deferens in the presence of abundant lipid vesicles, containing high concentrations of cholesterol and phospholipids (0.273 and 0.704nmole / μgDNA), these physiological lipid co-culture with sperm, can make Sperm loses activity. After washing with KRB solution, the sperm cholesterol and phospholipid contents were 0.235 and 0.694 nmole / μg DNA, respectively. Washing and further incubation with KRB-BSA had no significant effect on the sperm cholesterol and phospholipid content. Separation of sperm by two-step PGC method could be significantly reduced Sperm phospholipid content (0.315 nmole / μg DNA).