本文应用4对微卫星分子多态引物,对利用优株嫁接建立的蓝桉(E. globulus)树木园中的118个自由授粉子代的DNA微卫星指纹进行了分析。结果表明:选用的引物具有高度多态性,各个位点扩增得到的平均等位基因数为14.25,树木园中母株的异型杂交率为15-?95%,整体异型杂交率为47.9%,外源花粉污染率为17.6%。由于该树木园的异型杂交率低,其自由授粉的种子应用价值不高,因此应采用大规模人工授粉来制种或选用树木园中异型杂交率高的母株采种。 In this study, DNA microsatellite fingerprinting of 118 free-pollinated progeny in E. globulus arboretum was established by using four pairs of microsatellite loci polymorphic primers. The results showed that the selected primers were highly polymorphic, the average number of alleles amplified by each locus was 14.25, the abnormal hybridization rate of mother plants in arboretum was 15-95%, and the overall heterotypic hybridization rate was 47.9% , Exogenous pollen contamination rate was 17.6%. Due to the low rate of heterozygosis in this arboretum and its application of free pollinated seeds, the large-scale artificial pollination should be used to seed or choose the parental plant with abnormally shaped hybridization rate in the arboretum.