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建立不同产地盾叶薯蓣药材中甾体总皂苷成分HPLC-ELSD指纹图谱的方法。采用Welchrom C18(4.6 mm×250 mm,5μm)色谱柱,乙腈(A)-水(B)作为流动相,梯度洗脱;柱温室温;体积流速1.0 mL·min-1;蒸发光散射检测器条件:漂移管温度90.0℃,载气(N2)流速2.8 L·min-1;进样体积10μL。通过检测10个批次盾叶薯蓣药材,首次建立了盾叶薯蓣甾体总皂苷成分HPLC-ELSD指纹图谱的共有模式,确定了25共有峰,并利用对照品对照法对其中的10个共有峰进行了成分指认。以共有模式对10批药材进行相似度评价,其相似度全部大于0.80。该方法准确、可靠、重复性好,为全面评价和控制盾叶薯蓣药材的质量提供了科学依据。
Method for establishing HPLC-ELSD fingerprinting of steroidal total saponins in Dioscorea zingiberensis. The mobile phase was eluted with a gradient of acetonitrile (A) -water (B) using a column of Welchrom C18 (4.6 mm × 250 mm, 5 μm). The column temperature was at room temperature and the volume flow rate was 1.0 mL · min -1. Conditions: drift tube temperature 90.0 ℃, carrier gas (N2) flow rate 2.8 L · min-1; injection volume 10μL. By testing 10 batches of Dioscorea zingiberensis, the common mode of HPLC-ELSD fingerprinting of Dioscorea zingiberensis CHWright was established for the first time, 25 common peaks were identified and 10 common peaks The ingredients were identified. The similarity of 10 batches of medicinal herbs to the common mode was evaluated, and the similarity was all greater than 0.80. The method is accurate, reliable and reproducible, which provides a scientific basis for the comprehensive evaluation and control of the quality of Dioscorea zingiberensis.