论文部分内容阅读
目的建立适合实验室内测定人血浆中亚胺培南、美罗培南、帕尼培南、法罗培南4种特殊使用抗生素的通用高效液相色谱方法。方法采用互为内标法,血浆样品经乙腈沉淀蛋白,取上清加二氯甲烷萃取,涡旋离心后取上层进样分析。色谱柱为Kromasil 100-5 C18(4.6 mm×150 mm,5μm);流动相为甲醇-0.015 mol·L-1MOPS溶液(pH 7.3),梯度洗脱法;流速为0.5 mL·min-1;柱温30℃,检测波长299 nm。结果亚胺培南在1.56~200.00 mg·L-1内线性良好(r=1.000 0),美罗培南在1.56~100.00 mg·L-1内线性良好(r=0.999 5),帕尼培南在1.56~200.00 mg·L-1内线性良好(r=0.999 0),法罗培南在1.56~200.00 mg·L-1内线性良好(r=0.999 8)。4药低、中、高浓度(5.00,20.00,80.00 mg·L-1)的日内RSD为0.35%~4.09%(n=5),日间RSD为0.54%~7.34%(n=3),提取回收率为(75.48±10.52)%~(103.27±2.90)%(n=5),方法学回收率为(91.59±3.63)%~(113.10±5.22)%(n=5)。结论本方法灵敏度高,简便快捷,可满足临床血药浓度测定的需要。
OBJECTIVE To establish a universal HPLC method for the determination of 4 specific antibiotics in human plasma using imipenem, meropenem, panipenem and faropenem. Methods The internal standard method was used for each. The plasma samples were precipitated by acetonitrile, the supernatant was extracted with dichloromethane, and the supernatant was injected by vortexing for analysis. The column was Kromasil 100-5 C18 (4.6 mm × 150 mm, 5 μm). The mobile phase consisted of methanol-0.015 mol·L-1 M OPS solution (pH 7.3) with gradient elution at a flow rate of 0.5 mL · min -1. Temperature 30 ℃, detection wavelength 299 nm. Results Imipenem had a good linearity (r = 1.000 0) within 1.56-200.00 mg · L-1 and a good linearity within 1.56-100.00 mg · L-1 (r = 0.999 5) 1.56 ~ 200.00 mg · L-1 showed a good linearity (r = 0.999 0). Faropenem had good linearity (r = 0.999 8) within 1.56-200.00 mg · L-1. The intra-day RSD of low, medium and high concentrations of drug (5.00,20.00,80.00 mg · L-1) were 0.35% -4.09% (n = 5) and daytime RSD was 0.54% -7.34% The recovery rate was (75.48 ± 10.52)% ~ (103.27 ± 2.90)% (n = 5). The methodological recovery was 91.59 ± 3.63% ~ 113.10 ± 5.22%. Conclusion The method is sensitive, simple and quick, and can meet the needs of clinical blood concentration determination.