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目的 探讨供体特异性基因片段MHCClassI类抗原分子RT1.AacDNA在诱导免疫耐受中的作用和可能机制。方法 采用大鼠同种异体心脏异位移植模型 ,通过供体MHCClassI类抗原的RT1.AacDNA基因片段转染受体成肌细胞 (MB)并接种自体胸腺 ,观察移植物存活时间 ,判断受体免疫耐受产生和维持的状态。结果 经胸腺接种转染供体基因的自体成肌细胞并同时服用CsA ,移植物平均存活时间高达 ( 96 .13± 12 .91)d ,明显高于其它实验组 (P <0 .0 5 ) ;动态混合淋巴细胞反应 (MLR) ,无论外周输注或胸腺接种其对照组cpm值均高于各自实验组 ;CD4 +/CD8+比值更能反映机体的的免疫状态。结论 供者特异性MHCClassI类分子RT1.Aa 能诱导受体对供体心脏移植物的免疫耐受 ,作用持久 ;在诱导耐受中 ,RT1.Aa 的作用大于供者免疫活性细胞 (脾细胞 ) ;胸腺途径产生的中枢耐受效果优于外周途径
Objective To investigate the role and possible mechanism of donor-specific gene fragment MHC class I antigen (RT1AacDNA) in inducing immune tolerance. Methods Rat heterotopic cardiac allograft model was used to transfect recipient myoblasts (MB) into recipient myoblasts via RT1.AacDNA gene fragment of donor MHCC class I antigen. The graft survival time was observed and the receptor immunity Tolerance to produce and maintain the state. Results The average survival time of transplants was (96.13 ± 12.91) days, which was significantly higher than that of other experimental groups (P <0.05) ; Dynamic mixed lymphocyte reaction (MLR), regardless of peripheral infusion or thymus inoculation cpm value of the control group were higher than the respective experimental group; CD4 + / CD8 + ratio better reflect the body’s immune status. Conclusion The donor-specific MHC class I molecule RT1.Aa can induce the immune tolerance of donor recipients to donor heart allografts. The effect of RT1.Aa is greater than that of donor immunocompetent cells (spleen cells) The central tolerance produced by the thymus pathway is superior to the peripheral pathway