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利用 Ｒ Ｔ Ｐ Ｃ Ｒ 技术， 我们检测了 Ｐ１９ 细胞体外神经元分化过程中神经钙粘着蛋白（ Ｎｃａｄｈｅｒｉｎ） 的表达模式。结果显示， 该基因在上述过程中存在上调和下调过程， 与体内中枢神经系统发育过程的表达模式十分相近。在此基础上， 我们将神经钙粘着蛋白基因ｃ Ｄ Ｎ Ａ 全长转入 Ｐ１９ 细胞， 通过药物筛选， 得到了稳定表达神经钙粘着蛋白的细胞株。转基因细胞株不经 Ｒ Ａ 诱导， 就能自发向神经元方向分化， 并表达神经元特异的分子标记物 Ｎ Ｆ１６０ 。以上结果提示： 神经钙粘着蛋白可引起 Ｐ１９ 细胞在体外自发向神经方向分化， 在 Ｐ１９ 神经元的分化过程中起重要作用。
Using RT-PCR, we examined the expression of N-cadherin in P19 cells in vitro during neuronal differentiation. The results showed that there was up-regulation and down-regulation of this gene in the above process, which was similar to the expression pattern of CNS development in vivo. On this basis, we transferred the full-length cD N A of the neural cadherin gene into P19 cells and obtained the cell strain stably expressing the neural cadherin through drug screening. Transgenic cell lines can spontaneously differentiate into neurons without R A induction and express neuron-specific molecular marker N F160. These results suggest that: neuronal cadherin can cause P19 cells in vitro to spontaneous neural differentiation, P19 neurons play an important role in the process of differentiation.