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研究下调CDH17基因对那可丁耐药的人胃癌MGC-803细胞裸鼠移植瘤模型的影响,为临床上寻求治疗胃癌新方案提供理论依据。以人胃癌MGC-803细胞作为研究材料,建立该细胞的裸鼠移植瘤模型。同时,用HE染色法观察瘤体组织病理学形态。结果显示,RT-PCR和Western blotting实验中siCDH17慢病毒对胃癌MGC-803细胞中CDH17的转录水平和蛋白质表达水平均具有干扰效果(P<0.05)。随后将siCDH17慢病毒干扰的人胃癌MGC-803细胞以及对照细胞进行裸鼠皮下移植瘤实验。移植后第6天各组均见皮下肿瘤结块,采用那可丁药物处理并实时检测肿瘤生长情况。结果发现,siCDH17组肿瘤体积和质量明显小于对照组和siNC组(P<0.05)。且HE染色也发现与对照组比较,siCDH17组的瘤体肿瘤细胞生长受到抑制。因此,下调CDH17基因可以增加胃癌MGC-803细胞对那可丁的敏感性,抑制胃癌MGC-803细胞的生长,最终阻滞裸鼠移植瘤的生长。
To study the effect of downregulation of CDH17 gene on the nude mice model of gastric cancer cell line MGC-803 that is resistant to nadine, and to provide a theoretical basis for clinical treatment of gastric cancer. Using human gastric cancer cell line MGC-803 as a research material, a nude mouse xenograft model was established. At the same time, the histopathology of the tumor was observed by HE staining. The results showed that siCDH17 lentivirus had an interference effect on CDH17 transcription and protein expression level in gastric cancer MGC-803 cells by RT-PCR and Western blotting (P <0.05). Subsequently, human gastric cancer MGC-803 cells interfered by siCDH17 lentivirus as well as control cells were transplanted subcutaneously in nude mice. On the 6th day after transplantation, subcutaneous tumors were found in all groups. Naketin was used to treat the tumors and the tumor growth was detected in real time. The results showed that the tumor volume and quality of siCDH17 group was significantly smaller than that of the control group and siNC group (P <0.05). And HE staining also found that compared with the control group, siCDH17 tumor growth of tumor cells were inhibited. Therefore, downregulation of CDH17 gene can increase the sensitivity of gastric cancer MGC-803 cells to nocturnine, inhibit the growth of gastric cancer MGC-803 cells and eventually block the growth of xenografted tumors in nude mice.