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目的通过门静脉输注同种异基因脾细胞、腹腔给予环磷酰胺诱导同种心脏移植耐受 ,并探讨其耐受机理。方法经门静脉给予受体大鼠 3× 10 8个异基因供体脾细胞 ,2 d后腹腔注射 80 m g/kg的环磷酰胺 ,10 d后实施心脏移植手术。观察、记录移植物的存活时间 ,通过 ML R、DTH、IL- 2逆转实验及过继性转移实验 ,探讨耐受机理。结果异基因心脏移植物的存活时间明显延长 ;ML R和 DTH实验证明 :受体大鼠的免疫应答受抑制 ,且该耐受表现为供体特异性 ;IL- 2逆转实验、过继性转移实验表明 :该耐受与克隆失活、抑制细胞和“感染”耐受机理有关。结论本诱导方案对于大鼠心脏移植存活时间的延长是一有效的方法 ,移植耐受的形成涉及多种机制。
OBJECTIVE: Allogeneic splenocytes were infused via portal vein and intraperitoneally administered with cyclophosphamide to induce allograft tolerance, and to explore the mechanism of its tolerance. Methods 3 × 10 8 allogeneic donor spleen cells were injected via the portal vein. After 2 d, cyclophosphamide (80 m g / kg) was intraperitoneally injected. Heart transplantation was performed 10 days later. The survival time of the graft was observed and recorded, and the mechanism of tolerance was discussed by reversing ML R, DTH, IL-2 and adoptive transfer. Results Allogeneic heart transplantation significantly prolonged the survival time; ML R and DTH experiments showed that: the immune response of the recipient rats was inhibited, and the tolerance showed donor specificity; IL-2 reversal test, adoptive transfer test This indicates that this tolerance is associated with inactivation of clones, inhibition of cell and “infection” tolerance mechanisms. Conclusion This induction regimen is an effective method for prolonging the survival time of heart transplantation in rats. The formation of transplantation tolerance involves many mechanisms.