本文由美国某综合医院在1979年10月～1981年5月对758例3岁以下儿童进行了A 群乙型溶血性链球菌(GABHS)致上呼吸道感染病例的调查,主要采集鼻拭子、咽拭子、炎性分泌物或脓汁标本接种于含胰蛋白酶的5%羊血琼脂(TSBAP)平板,同时接种于一种选择性Q 培养基中,置37℃厌氧培养,Q 培养基的阳性者再转种于TSBAP上,于24、48小时观察菌落,挑取典型β溶血的菌落作 This article from the United States in a general hospital from October 1979 to May 1981 758 cases of children under the age of 3 A group B hemolytic streptococcus (GABHS) caused by upper respiratory tract infection in the investigation, the main collection of nasal swabs, Throat swabs, inflammatory secretions or pus samples were inoculated on trypsin-containing 5% sheep blood agar (TSBAP) plates and seeded in a selective Q medium at 37 ° C in anaerobic culture, Q medium Were transplanted on TSBAP again, and colonies were observed at 24 and 48 hours to pick out typical β hemolysis colonies