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目的观察维生素C(VC)、维生素E(VE)对体外培养H2O2氧化损伤血管内皮细胞(VEC)超微结构的影响。方法采用体外细胞培养方法,将血管内皮细胞分为正常对照组,H2O2氧化损伤对照组、VC+H2O2组(VC100μmol/L),VE+H2O2组(VE100μmol/L)。分别将VC、VE加入到培养液中,预孵24h,再加入除菌后的H2O2进行损伤,终止培养后收集细胞,进行电镜观察。结果透射电镜观察VEC,可见H2O2损伤对照组细胞超微结构明显异常,细胞表面呈光滑状,丢失特征性的细胞突起及微绒毛;胞质内线粒体损伤严重,形态模糊;粗面内质网明显扩张呈大囊泡状;溶酶体增多;少数细胞严重水肿,核形不规则,核内染色质部分溶解。VC+H2O2和VE+H2O2组血管内皮细胞超微结构仅有轻度损伤,多数细胞与正常对照组细胞相似;大部分细胞表面微绒毛和胞质突起均趋于正常,胞质内细胞器均在正常范围内;细胞核未见异常,核膜完好,核内染色质分布正常;仅有少数细胞粗面内质网有轻度扩张,线粒体结构模糊不清。结论VC、VE可一定程度保护氧化损伤的血管内皮细胞的超微结构。
Objective To observe the effects of vitamin C (VC) and vitamin E (VE) on the ultrastructure of vascular endothelial cells (VEC) injured by H2O2 in vitro. Methods Vascular endothelial cells were divided into normal control group, H2O2 oxidative damage control group, VC + H2O2 group (VC100μmol / L), VE + H2O2 group (VE100μmol / L) by in vitro cell culture method. VC and VE were respectively added to the culture solution for 24 h preincubation, followed by addition of H2O2 after sterilization. Cells were harvested after culture was stopped and observed under electron microscope. Results VEC was observed by transmission electron microscopy. The ultrastructure of H2O2-injured cells in the control group was obviously abnormal. The cell surface was smooth and the characteristic cell protrusions and microvilli were lost. The mitochondria in the cytoplasm were severely damaged and the morphology was rough. The rough endoplasmic reticulum Expansion was large vesicular; lysosome increased; a few serious cell edema, nuclear irregular, chromatin chromatin partially dissolved. The ultrastructures of vascular endothelial cells in VC + H2O2 and VE + H2O2 groups were only slightly damaged, most of the cells were similar to the normal control cells; most of the microvilli and cytoplasmic processes tended to be normal in the cells, Within the normal range; no abnormalities in the nucleus, intact nuclear membrane, normal distribution of chromatin in the nucleus; slight expansion of the rough endoplasmic reticulum in only a few cells, mitochondrial structure blurred. Conclusion VC and VE can protect the ultrastructure of oxidative injured vascular endothelial cells to some extent.