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目的:建立反相高效液相色谱法测定祛瘀清胰颗粒中牛磺熊去氧胆酸含量的方法。方法:采用高效液相色谱法。色谱柱:Agilent TC-C18(4.6 mm×250 mm,5μm);流动相乙腈-0.5%磷酸水溶液,梯度洗脱;检测波长203nm;流速1.0 mL.min-1;柱温30℃。结果:牛磺熊去氧胆酸在0.259～5.180μg范围内线性关系良好,r=0.9999,平均回收率99.2%,RSD为2.3%。结论:本方法简便、准确、灵敏度高,重复性好,可有效控制祛瘀清胰颗粒的质量。
Objective: To establish a method for the determination of tauroursodeoxycholic acid in Quyu Qingyi Granules by reversed-phase high performance liquid chromatography. Methods: Using high performance liquid chromatography. Column: Agilent TC-C18 (4.6 mm × 250 mm, 5 μm); mobile phase of acetonitrile-0.5% phosphoric acid aqueous solution, gradient elution; detection wavelength of 203 nm; flow rate of 1.0 mL.min-1; Results: There was a good linear relationship between tauroursodeoxycholic acid and 0.259-5.180μg, r = 0.9999, the average recovery was 99.2% and the RSD was 2.3%. Conclusion: The method is simple, accurate, sensitive and reproducible, which can effectively control the quality of Quyu Qingyitao granules.