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目的观察POU5fl转录因子-4(OCT4)及性别决定基因相关转录因子-2(SOX2)在胃癌组织表达的变化及其与胃癌病理特征的相关性。方法 48例胃癌组织作为研究对象,48例胃正常组织作为对照(距离肿瘤病灶>10 cm)。利用免疫组化检测手段比较OCT4及SOX2在胃癌组织及胃正常组织的变化,分析其与胃癌各病理指标的相关性。结果 OCT4及SOX2在胃癌组织阳性表达率分别为75.0%及25.0%,胃正常组织分别为27.1%及64.6%,癌组织与正常组织比较差异均有统计学意义(P<0.005);OCT4与SOX2蛋白在癌组织表达呈负相关性(r=-3.29,P=0.002<0.05)。经过分析后发现OCT4在胃癌组织的表达与淋巴结转移和TNM分期及分化程度有关(P<0.05),SOX2在胃癌组织的表达与胃癌的TNM分期和淋巴结转移及分化程度有关(P<0.05)。结论胃癌的发生可能与OCT4升高、SOX2的下调有关,OCT4及SOX2的联合检测可作为预测胃癌发展的靶指标。
Objective To observe the expression of POU5fl transcription factor-4 (OCT4) and sex-determining gene-related transcription factor-2 (SOX2) in gastric cancer tissues and their correlation with gastric cancer pathological features. Methods Forty-eight patients with gastric cancer were used as the study subjects. Forty-eight normal gastric tissues were used as controls (distance from tumor lesions >10 cm). The changes of OCT4 and SOX2 in gastric cancer tissues and normal gastric tissues were compared by immunohistochemical detection methods, and their correlation with gastric cancer pathological parameters was analyzed. Results The positive expression rates of OCT4 and SOX2 in gastric cancer tissues were 75.0% and 25.0%, respectively. The normal tissues in stomach were 27.1% and 64.6% respectively. The difference between cancer tissues and normal tissues was statistically significant (P<0.005); OCT4 and SOX2 Protein expression was negatively correlated in cancer tissues (r=-3.29, P=0.002<0.05). After analysis, it was found that the expression of OCT4 in gastric cancer was related to lymph node metastasis and TNM stage and differentiation (P<0.05). The expression of SOX2 in gastric cancer tissues was related to TNM stage and lymph node metastasis and differentiation of gastric cancer (P<0.05). Conclusion The occurrence of gastric cancer may be related to the increase of OCT4 and the down-regulation of SOX2. The combined detection of OCT4 and SOX2 may serve as a target for predicting the development of gastric cancer.