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目的钻研高原肺水肿患者血浆中一氧化氮浓度改变;研究高原肺水肿患者血浆一氧化氮合酶和鸟氨酸脱羧酶酶活力和蛋白表达变化,及其血清蛋白浓度与肺动脉高压的相关性研究。经此研究,探究一氧化氮合酶和鸟氨酸脱羧酶对高原肺水肿发生和发展的作用。方法通过对30例高原肺水肿患者血浆中一氧化氮浓度、一氧化氮合酶和鸟氨酸脱羧酶活力和蛋白表达进行检测,并与30例正常人进行差异性比较。结果高原肺水肿患者血浆中一氧化氮水平显著下降(P<0.01);一氧化氮合酶显著降低(P<0.01);鸟氨酸脱羧酶酶活力较正常人没有差异(P>0.01),但是有上升的趋势;鸟氨酸脱羧酶显著升高(P<0.01);高原肺水肿患者肺动脉压与血浆中鸟氨酸脱羧酶含量呈负相关(P<0.05)。结论一氧化氮和一氧化氮合酶在高原肺水肿的发生和发展起着重要的调节作用;鸟氨酸脱羧酶在高原肺水肿患者血浆中的高表达可能对受损组织起到修复作用。
To study the changes of plasma nitric oxide concentration in patients with pulmonary edema in highland and to study the changes of plasma nitric oxide synthase and ornithine decarboxylase activity and protein expression in patients with high altitude pulmonary edema and the correlation between serum protein concentration and pulmonary hypertension . After this study, to explore nitric oxide synthase and ornithine decarboxylase on the occurrence and development of high altitude pulmonary edema. Methods The levels of nitric oxide, nitric oxide synthase and ornithine decarboxylase (NO) in plasma and protein expression of 30 patients with pulmonary edema were detected and compared with those of 30 normal subjects. Results The level of NO in plasma of patients with high altitude pulmonary edema decreased significantly (P <0.01), the level of nitric oxide synthase decreased significantly (P <0.01), and the activity of ornithine decarboxylase was no significant difference (P> 0.01) But there was an upward trend. The ornithine decarboxylase was significantly increased (P <0.01). The pulmonary arterial pressure was negatively correlated with the content of ornithine decarboxylase (P <0.05) in patients with high altitude pulmonary edema. Conclusions Nitric oxide and nitric oxide synthase play an important regulatory role in the pathogenesis and progression of high altitude pulmonary edema. Overexpression of ornithine decarboxylase in the plasma of patients with high altitude pulmonary edema may repair the damaged tissue.