论文部分内容阅读
目的 :检测 5种多药耐药基因在乳腺癌细胞株 MCF- 7和 MCF- 7/ADR里的表达强度变化 ,为乳腺癌多药耐药逆转研究提供新的思路。方法 :通过实时荧光定量 PCR技术分别检测乳腺癌敏感细胞株 MCF- 7和耐药细胞株 MCF- 7/ADR里的 mdr1、MRP、L RP、GST- π、TOPO α基因表达强度。结果 :MCF- 7/ADR里的 mdr1、MRP、LRP、GST- π基因表达强度分别比它们在 MCF- 7里的表达强度相对增加了 1 2 5 .39、5 5 .5 3、1 .2 4、3.38倍 ,TOPO α则降低了 0 .43倍 ,其中 mdr1、MRP表达强度的相对增加倍数均显著高于 L RP、GST- π、TOPO α的增加 (或降低 )倍数 ( P<0 .0 0 1 )。结论 :除了 mdr1之外 ,MRP也可能在乳腺癌的多药耐药产生机制里起重要作用。因此 MRP基因可以成为乳腺癌耐药逆转研究的新作用靶点
Objective: To detect the expression intensity of five multidrug resistance genes in breast cancer cell lines MCF-7 and MCF-7 / ADR and to provide new ideas for the study of multidrug resistance reversal in breast cancer. Methods: The expression of mdr1, MRP, LRP, GST-π and TOPO α in breast cancer cell lines MCF-7 and MCF-7 / ADR were detected by real-time fluorescence quantitative PCR. Results: The expression intensity of mdr1, MRP, LRP and GST-π in MCF-7 / ADR were respectively increased by 125%, 39.5% and 53.1% 4,3.38-fold, and TOPO α decreased by 0.43-fold. The relative increase of mdr1 and MRP was significantly higher than that of L RP, GST-π and TOPO α (P <0. 0 0 1). Conclusion: In addition to mdr1, MRP may play an important role in the multidrug resistance mechanism of breast cancer. Therefore, MRP gene can be a new target of reversal of drug resistance in breast cancer