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目的:探索简捷细胞培养方法并观察该方法对癌细胞特性的影响。方法:将复苏的OC-3-VGH卵巢癌细胞株不经洗涤直接移至细胞培养瓶,加入10ml RPMI-1640培养液,放入培养箱,余同传统方法,观察细胞生长情况;取细胞悬液,以细胞数4×106/ml,0.2ml/只接种至BALB/c雌性裸小鼠皮下,2个月后处死,取肿瘤组织制片,并与传统培养方法结果进行比较。结果:两种方法培养的细胞均生长活跃,皮下接种7d左右,裸鼠长出肿瘤,组织学检查未见明显差异。结论:改良细胞培养方法减少了传统方法的繁琐步骤,方便细胞培养。
Objective: To explore the method of cell culture and to observe the effect of this method on the characteristics of cancer cells. Methods: The resuscitated OC-3-VGH ovarian cancer cell line was directly transferred to the cell culture flask without washing, added with 10ml RPMI-1640 culture medium and placed in an incubator, the same as the traditional method to observe the cell growth; The cells were inoculated into BALB / c female nude mice subcutaneously at the dose of 4 × 106 / ml and 0.2 ml / ml respectively. After 2 months, the mice were sacrificed and the tumor tissues were prepared and compared with the results of traditional culture methods. Results: The cells cultured by both methods all grew actively. After being inoculated subcutaneously for about 7 days, tumors grew in nude mice and there was no obvious difference in histological examination. Conclusion: Improved cell culture methods reduce the cumbersome steps of traditional methods and facilitate cell culture.