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目的 :采用由脐带血血清中提纯的胎蛋白 (Alpha-fetoprotein,AFP)作用于体外培养的Hela细胞。方法 :通过对MTT计数 ,3H -TdR掺入以及细胞周期时相改变等指标的检测。结果 :发现AFP(浓度大于10mg/L)对Hela细胞增殖有明显的促进作用。结论 :AFP具有促Hela细胞增殖的生理功能
OBJECTIVE: To study the effects of Alpha-fetoprotein (AFP) purified from umbilical cord serum on cultured Hela cells. Methods: MTT count, incorporation of 3H-TdR and cell cycle phase changes and other indicators of detection. Results: It was found that AFP (concentration> 10mg / L) significantly promoted the proliferation of Hela cells. Conclusion: AFP has the physiological function of promoting the proliferation of Hela cells