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目的利用彗星实验和微核实验反映土壤有机污染物的联合毒性。方法选取某污灌农田土壤为研究对象(下称“污灌区”),地下水灌溉农田土壤为对照(下称“对照区”),采用超声振荡法提取土壤中的有机污染物,用气相色谱质谱联用仪(GC/MS)对土壤中的有机物进行分析;采用灌胃的方式对小鼠进行染毒,用单细胞凝胶电泳实验和微核试验检测土壤有机提取物的联合毒性。结果污灌区土壤检出的主要污染物为烷烃类、苯系物及多环芳烃,有机污染物的种类及含量高出对照区数倍;与试剂对照组和对照区相同染毒剂量组比较,污灌区各组拖尾率、微核率均增高(P﹤0.05)。结论该污灌区土壤已受到一定程度的有机物污染,且污染物对小鼠具有一定的毒性。
Objective To use the comet assay and micronucleus test to reflect the combined toxicity of soil organic pollutants. Methods Soil of a sewage irrigation farmland was selected as the research object (hereinafter referred to as “sewage irrigation area”) and groundwater irrigated farmland soil was used as a control (hereinafter referred to as “control area”). Ultrasonic oscillation was used to extract organic pollutants from the soil, The organic compounds in the soil were analyzed by gas chromatography-mass spectrometry (GC / MS). The mice were inoculated intragastrically, and the combination of soil organic extracts was detected by single cell gel electrophoresis and micronucleus test toxicity. Results The main contaminants detected in soils of sewage irrigation area were alkanes, benzene compounds and polycyclic aromatic hydrocarbons, and the types and contents of organic pollutants were several times higher than that of the control area. Compared with the same dose of the control and control groups, The tailing rate and micronucleus rate of each group in sewage irrigation area were increased (P <0.05). Conclusion The soil in the sewage irrigation area has been contaminated by some organic compounds, and the pollutants have certain toxicity to mice.