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目的:建立成年人脑皮质神经干细胞系,为临床自体移植治疗脑损伤提供资料。方法:实验于2002-09/2004-05在郑州大学第一附属医院神经外科干细胞中心实验室进行,外科手术获得成年人脑皮质碎片(已签定自愿同意书),利用无血清培养技术培养神经干细胞,采用免疫细胞化学技术进行鉴定。结果:部分成人脑皮质细胞,在体外分裂增殖形成细胞球,能进行连续传代,并分化产生中枢神经系统的3种主要类型细胞:具有一个或两个长突起、胞体呈圆形或椭圆形的神经元样细胞,具有多个粗大突起、分支较多而胞体欠规整的星形胶质样细胞,具有多个细长突起、分支较少而胞体小且规整的少突胶质样细胞。免疫细胞化学显示分别具有神经元特异性烯醇化酶、胶质纤维酸性蛋白。结论:成人脑皮质的部分细胞,具有自我更新、增殖和多向分化能力。
OBJECTIVE: To establish an adult human neural cortex neural stem cell line to provide data for clinical autologous transplantation for brain injury. Methods: The experiment was performed in the Department of Neurosurgery Stem Cell Central Laboratory, the First Affiliated Hospital of Zhengzhou University from September to May 2004, and obtained the cerebral cortex fragments in adults (signed the voluntary consent form). The nerve cells were cultured with serum-free culture Stem cells were identified by immunocytochemistry. RESULTS: Some adult human cortical cells proliferated in vitro to form cytoplasmic spheres, which could be continuously passaged and differentiated into three major types of central nervous system cells: one or two long protuberances with a round or oval cell body Neuron-like cells, astrocyte-like cells with numerous bulky protrusions, more branches and less well-organized cells, oligodendrocyte-like cells with many elongated protrusions, few branches, and a small cell body. Immunocytochemistry showed neuron-specific enolase and glial fibrillary acidic protein, respectively. Conclusion: Some cells of adult cerebral cortex have the ability of self-renewal, proliferation and multidirectional differentiation.