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本研究探讨了咖啡因代谢物在Ｎ－乙酰化酶活性评价中的意义。用ＨＰＬＣ法测定咖啡因代谢物，所有代谢物均能良好分离。以５－乙酰胺基－６－甲酰胺基－３－甲基尿嘧啶（ＡＦＭＵ）和１－甲基黄嘌呤（１Ｘ）摩尔浓度比值作为评价Ｎ－乙酰化酶的代谢指标。对１２０名志愿者进行了Ｎ－乙酰化酶多态性分析，受试者可明显划分为快乙酰化和慢乙酰化者，快、慢乙酰化表型之比为１００：２０。与同时用磺胺二甲嘧啶作为探针进行乙酰化分型的结果一致。因此以咖啡因作为探针评价人体Ｎ－乙酰化酶活性，不失为一种可行、简便的方法。
This study explored the significance of caffeine metabolites in the evaluation of N-acetylase activity. Determination of caffeine metabolites by HPLC, all metabolites were well separated. The metabolic ratio of 5-acetamido-6-formamido-3-methyluracil (AFMU) and 1-methylxanthine (1X) was used as a metabolic index to evaluate N-acetylase. N-acetyl-amylase polymorphism was analyzed in 120 volunteers. Subjects were clearly divided into fast acetylated and slow acetylated. The ratio of fast and slow acetylated phenotypes was 100: 20. Consistent with the results of acetylation typing with sulfamethazine as probe at the same time. Therefore, caffeine as a probe to evaluate human N-acetylase activity, after all, a feasible and easy way.