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目的探讨p53基因、碱性成纤维生长因子(b FGF)和微血管密度(MVD)检测用于垂体腺瘤侵袭性判断以及评估预后的临床价值。方法选取2009年3月至2014年3月收治的40例垂体腺瘤患者,按其手术后病理诊断结果分为侵袭组和非侵袭组,应用免疫组化方法检测p53基因和b FGF表达情况,对肿瘤组织内的MVD进行标记并计数;通过逆转录聚合酶链反应(RT-PCR)对p53和b FGF的mRNA表达情况进行检测,分析侵袭组和非侵袭组间p53、b FGF和MVD的表达差异。结果侵袭组的p53和b FGF蛋白及mRNA表达水平相较非侵袭组均明显升高,差异有统计学意义(P<0.05)。侵袭组的MVD计数较非侵袭组也有显著提高,差异有统计学意义(P<0.05)。Pearson相关分析结果显示,侵袭组中,p53与b FGF的mRNA表达水平正相关(r=0.61,P<0.05);p53 mRNA的表达与MVD计数正相关(r=0.68,P<0.05);b FGF的mRNA表达水平与MVD计数正相关(r=0.82,P<0.05)。结论在垂体腺瘤的侵袭性判断以及预后评估方面,p53、b FGF和MVD是比较可靠的生物学指标,可用于临床诊治和预后参照。
Objective To investigate the clinical value of p53 gene, basic fibroblast growth factor (b FGF) and microvessel density (MVD) in evaluating the invasiveness of pituitary adenomas and assessing prognosis. Methods Forty patients with pituitary adenoma who were treated from March 2009 to March 2014 were divided into invasive group and noninvasive group according to the postoperative pathological diagnosis results. The expression of p53 gene and b FGF protein were detected by immunohistochemistry, The MVD in tumor tissues was marked and counted. The mRNA expression of p53 and b FGF was detected by RT-PCR. The expression of p53, b FGF and MVD in invasive and non-invasive groups Differences in expression. Results The expressions of p53 and b FGF protein and mRNA in invasive group were significantly higher than those in non-invasive group (P <0.05). The MVD counts in invasive group were significantly higher than those in non-invasive group (P <0.05). Pearson correlation analysis showed that there was a positive correlation between p53 and bFGF mRNA expression (r = 0.61, P <0.05); p53 mRNA expression was positively correlated with MVD (r = 0.68, P <0.05) FGF mRNA expression was positively correlated with MVD (r = 0.82, P <0.05). Conclusions The p53, b FGF and MVD are reliable biomarkers in the assessment of invasiveness and prognosis of pituitary adenoma. They can be used in clinical diagnosis and prognosis.