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为探讨倍硫磷(FEN)对神经系统毒作用的机理,作者研究了长期接触倍硫磷对海马的形态学和生化学作用。作者分别采用2月龄(幼龄组)及12月龄(成年组)雄性Long-Evans大鼠,经背部皮下给药,每周三次,分别染毒2个月及10个月。染毒结束后,称重动物,采用多聚甲醛心脏灌注法固定脑组织,脑组织横切,以甲苯紫或HE染色。部分组织做冰冻切片,用Friedenwald-Koelle法进行胆碱酯酶(AchE)染色。用过氧化物酶-抗过氧化物酶法进行神经胶质纤维蛋白(GFAP)
To explore the mechanism of the toxicity of fenthion (FEN) on the nervous system, the authors studied the morphological and biochemical effects of long-term exposure to fenthil on the hippocampus. The authors used male Long-Evans rats aged 2 months (young group) and 12-month old (adult group), respectively, subcutaneously on the back three times a week for 2 months and 10 months respectively. After the end of the exposure, the animals were weighed, and the brain tissue was fixed with paraformaldehyde cardiac perfusion method. The brain tissues were transected and the cells were stained with toluene violet or HE. Some tissues were frozen sections and Cholinesterase (AchE) staining was performed using the Friedenwald-Koelle method. Peroxidase-anti-peroxidase method for glial fibrin (GFAP)