人工锌指核酸酶的设计与应用

来源 :畜牧与兽医 | 被引量 : 0次 | 上传用户：gloria_yan

【摘要】

：

锌指核酸酶是一种人工合成的融合蛋白,由特异性识别靶DNA的锌指蛋白结构域连接非特异性的FokI切割结构域构造而成,能够在预定的DNA靶位点引起DNA双链中断,随后依靠非同源末端

【作者】

：

邓廷贤庞春英杨炳壮梁贤威

【机构】

：

中国农业科学院广西水牛研究所

【出处】

：

畜牧与兽医

【发表日期】

：

2004年期

【关键词】

：

锌指蛋白锌指核酸酶基因修饰基因治疗

【基金项目】

：

科技部国际科技交流与合作专项;

下载到本地 , 更方便阅读

下载此文赞助VIP

声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架

论文部分内容阅读

锌指核酸酶是一种人工合成的融合蛋白,由特异性识别靶DNA的锌指蛋白结构域连接非特异性的FokI切割结构域构造而成,能够在预定的DNA靶位点引起DNA双链中断,随后依靠非同源末端连接和同源重组修复两种途径修饰该位点.近些年来,该技术已广泛的应用于基因转变、基因修饰和基因治疗等方面的研究.本文将简要介绍锌指核酸酶结构与原理,重点对锌指核酸酶的设计和应用进行了回顾与展望.

其他文献

劣质水灌溉对土壤盐碱化及作物产量的影响

长期的劣质水灌溉将导致土壤潜在的次生盐碱化.通过在以色列的大田试验,分析了劣质水灌溉对浅埋地下水位条件下土壤盐碱动态和作物产量的影响.试验在安装有暗管排水系统的试

期刊

劣质水灌溉土壤盐碱化作物产量地下水位

Genotype-dependent activation or repression of HBV enhancer Ⅱ by transcription factor COUP-TF1

AIM: To study the expression of HBV enhancer Ⅱ by transcription factor COUP-TF1.METHODS: In order to study the regulation of HBV variants in the vicinity of th

期刊

Hepatitis B virusHepatitis B virus x proteinCOUP-TF1GenotypeEnhancer Ⅱ

Small bowel anisakiosis: A report of two cases

Small bowel stenosis is a serious complication of intestinal anisakiosis. The aim of this report is to investigate whether severe stenosis of the small intestin

期刊

AnisakiosisSmall intestine

Effects of Boron, Zinc, and Iron on the Gentiopicroside Content and Yield of Gentian

A field experiment of 2-year-old gentian (Gentiana manshurica Kitag.) with application of boron (B), zinc (Zn),and iron (Fe) in Taikang County, Heilongjiang Pro

期刊

BFegentiangentiopicrosideZn

Short mucin 6 alleles are associated with H pylori infection

AIM: To investigate the relationship between mucin 6(MUC6) VNTR length and H pylori infection.METHODS: Blood samples were collected from patients visiting the C

期刊

H pyloriMucin 6PolymorphismVariable number of tandem repeats

Cloning and Expression of Low Molecular Weight Glutenin Genes from the Chinese Elite Wheat Cultivar

The low molecular weight (LMW) glutenin subunits account for 40% of wheat gluten protein content by mass and these proteins are considered to significantly affe

期刊

low molecular weight glutenin subunitM-type low molecular weight glutenin gene

Residual Characteristics of Organochlorine Pesticides in Lou Soils with Different Fertilization Mode

Soil samples with three fertilization treatments (no fertilizer, corn straw and farm manure) collected from a Lou soil(Eum-orthic Anthrosol classfied using Chin

期刊

Lou soilfertilization modeorganochlorine pesticidesresidue

Effect of universal salt iodization on antithyroid drugs

Iodine deficiency disease (IDD) is common in China.An universal salt iodization (USI) program has been implemented by the Chinese government since 1996. As a re

期刊

iodine deficiency diseaseuniversal salt iodizationantithyroid drugs

Depression of biofilm formation and antibiotic resistance by sarA disruption in Staphylococcus epide

AIM: To study the effects of disruption of sarA gene on biofilm formation and antibiotic resistance of Staphylococcus epidermidis (S. epidermidis ).METHODS: In

期刊

sarABiofilmAntibiotic resistanceStaphylococcus epidermidis

气体扩散电极中三相交界线的研究

对气体扩散电极三相反应区域的基本单元简化得到了液滴电极模型,并建立了液滴电极的数学模型.对液滴电极数学模型求解,得出液滴电极在大电流输出工作条件下输出电流与三相反

期刊

气体扩散电极润湿角三相交界线

人工锌指核酸酶的设计与应用

与本文相关的学术论文