【摘 要】
：
OBJECTIVE To evaluate the effects of flavonoids from Xindakang (Hippophae Fructus flavone) on myo?cardial systolic and diastolic functions of isolated frog hearts and explore the possible mechanism, and provide experi?mental basis for improving the effect
【机 构】
：
Engineering Research Center of Tibetan Medicine Testing Technology of Ministry of Education,Tibetan
论文部分内容阅读
OBJECTIVE To evaluate the effects of flavonoids from Xindakang (Hippophae Fructus flavone) on myo?cardial systolic and diastolic functions of isolated frog hearts and explore the possible mechanism, and provide experi?mental basis for improving the effect and efficacy of Xindakang on cardiac function. METHODS The isolated frog heart perfusion specimens were prepared by Yagi\'s method, and the effects of different concentrations of Xindakang on myo?cardial contractility (0.0125, 0.025, 0.05, 0.1 and 0.2 g·L-1), heart rate and cardiac output of isolated frog heart were stud?ied. Acetylcholine, atropine and epinephrine were administered successively to analyze the effects of Xindakang on car?diac systolic function of isolated frogs under the action of different drugs, and compared with propranolol. The effect of extracellular calcium ion concentration on the action of Xindakang was studied by using low calcium concentration, high cal?cium concentration and normal Ren\'s solution. To study the effect and possible mechanism of Xindakang on cardiac systolic function of frog. RESULTS The concentration of Xindakang in the range of 0.0125-0.1 g·L-1 could weaken the contractility of isolated frog heart and increase the concentration of Xindakang. The inhibitory effect of Xindakang on con?tractility of isolated frog heart was enhanced, and showed obvious dose-effect relationship. Cardiac output was signifi?cantly decreased by Xindakang (P<0.01), slow heart rate (P<0.05); M receptor blocker atropine could not antagonize the contractile effect of Xindakang, and Xindakang could not completely antagonize the contractile effect of adrenalin. Xindakang could inhibit the isolated frog heart in low calcium concentration, high calcium concentration and normal Ren\'s solution, and increased with the increase of extracellular calcium concentration (P<0.01). CONCLUSION Xinda?kang has inhibitory effect on isolated frog heart, which may be achieved by blocking the calcium channel on myocardial cell membrane and reducing the calcium concentration in myocardial cells.
其他文献
OBJECTIVE To investigate the pharmacological effect and mechanism of Sanguisorba officinalis L. (SOL) in non-small cell lung cancer (NSCLC) in vitro and in vivo based on network pharmacology. METHODS Network pharmacology was used to analyze the improving
目的:对比分析我国与欧盟、美国、日本药品质量抽查检验制度(以下均简称“药品抽检制度”),为完善相关制度提供经验借鉴.方法:汇总各国药品抽检制度,从抽检目的、主要法规与组织框架、品种遴选、抽样与检验以及结果公告与处理方面进行对比分析.结果 与结论:在抽检目的方面各国抽检目的均相似,包括验证药品上市后质量等;在主要法规与组织框架方面中国与美国更为类似,均是管理部门负责组织工作,技术部门负责组织实施;在品种遴选方面,美国和欧盟均将风险评估放置重要位置,我国尚未充分利用此定量化方式确定抽检品种;在抽样与检验方面,
在对国内外关于药品工艺验证有关法规指南分析的基础上,明确了工艺验证的基本原则与要求.通过对近几年国内外药品检查中发现的药品生产企业在工艺验证方面存在缺陷的分析,对工艺验证中的常见问题进行总结、分类与提炼.为我国制药行业进一步做好工艺验证提供参考,同时也为工艺验证的检查提供借鉴.
OBJECTIVE Interleukin (IL)-1β, one of the principal inflammatory cytokines mainly secreted by mono?cytes and macrophages, is produced by cleavage of the inactive pro-IL-1βprecursor by caspase-1 via the NLRP3 inflam?masome complex. The fruits of Garcinia c
OBJECTIVE Aryl hydrocarbon receptor (Ahr) is thought to be a crucial factor that regulates immune responses, which may be involved in the pathogenesis of autoimmune inflammation including rheumatoid arthritis (RA). The results of our group in recent years
2021年8月18日,加拿大卫生部对瑞德西韦(商品名:Veklury)窦性心动过缓的潜在风险进行评估,得出结论认为瑞德西韦的使用与窦性心动过缓风险之间可能存在联系.加拿大卫生部将与Veklury制造商合作,更新加拿大产品安全性信息,以包括窦性心动过缓的潜在风险.
目的:探讨西酞普兰联合阿立哌唑治疗中度至重度抑郁症疗效及安全性.方法:选取2018年12月至2019年12月于我院接受治疗的80例患有中重度抑郁症患者,依据随机抽签法分为观察组(40例)与对照组(40例).观察组予以西酞普兰联合阿立哌唑治疗,对照组予以单纯西酞普兰治疗.对比两组HDRS评分、疗效、不良事件.结果:观察组HDRS评分在从基线到第2周(8.10±1.35)、从基线到第4周(12.59±1.80)、从基线到第6周(15.49±1.74)均明显大于对照组HDRS评分(P0.05).观察组早期改善的
目的:评价三氧自体血回输疗法(O3-AHT)治疗痛风的安全性和有效性.方法:检索PubMed,EmBase,Web of Science(WOS),The Cochrane Library,中国生物医学文献服务系统(CBM),维普(VIP),中国知网(CNKI)和万方数据知识服务平台(WanFang Data).利用EndNote X8软件管理文献,由两名研究人员根据纳入、排除标准独立进行文献筛选,提取数据,采用Cochrane质量评估工具评估偏倚风险.利用RevMan 5.3软件进行统计分析.结果:共纳
目的:系统评价替罗非班用于颅内动脉瘤围手术期的安全性和有效性,为临床合理用药提供循证参考.方法:计算机检索Cochrane图书馆、PubMed、Embase、中国生物医学、中国知网、维普、万方数据库,收集不同治疗方式下替罗非班联合或不联合双抗药物(试验组)对比双抗药物(对照组)治疗颅内动脉瘤的临床研究.筛选文献并提取资料,采用Cochrane系统评价随机对照试验(RCT)文献质量,采用纽卡斯尔-渥太华量表评价队列研究文献质量.采用RevMan 5.3软件进行Meta分析.结果:共纳入16项研究,其中8项为
马兜铃酸广泛存在于马兜铃科马兜铃属及细辛属等植物中,自上世纪90年代“马兜铃酸肾病”事件以来,因其肾毒性、肝毒性及致突变性引起社会关注.本文从实验室研究、临床研究、毒理机制和减毒研究四个方面,探讨近年来马兜铃酸毒副作用研究现状,为安全使用含马兜铃酸类药物提供参考.