血液碳氧血红蛋白的分析方法很多。为研制急性一氧化碳中毒诊断标准,需选择一种准确度高,且又简单方便,适于推广的方法。经过比较,选择了双波长分光光度法(432/420nm)。本文对此作了评价,经实际测定,并对其操作要点作了详细介绍。本法用血量少(5—10μl),操作省时,准确度与灵敏度均能满足临床与人群调查分析的要求。 Blood carboxyhemoglobin analysis of many ways. To develop a diagnostic criteria for acute carbon monoxide poisoning, the need to choose a high accuracy, but also simple and convenient, suitable for the promotion of the method. After comparison, dual wavelength spectrophotometry (432 / 420nm) was chosen. This article made an evaluation, after the actual determination, and the operation of the key points were introduced in detail. This law with less blood (5-10μl), operation time-saving, accuracy and sensitivity can meet the clinical and population survey and analysis requirements.