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目的探讨超短波、旋磁治疗对大鼠局灶性脑缺血再灌注损伤的影响。方法应用线栓法制作大鼠局灶性脑缺血再灌注模型。将52只健康Wistar大鼠随机分为假手术组、对照组、超短波治疗组(超短波组)及旋磁治疗组(旋磁组)。脑缺血再灌注后18h给予治疗,24h取脑,观察各组大鼠脑组织含水量、脑梗死体积及Bcl-2、Bax蛋白表达,进行组间比较。结果脑缺血再灌注后18h,超短波治疗能减少脑含水量,减小梗死体积;超短波组与旋磁组Bcl-2的表达均提高,Bax蛋白表达降低,Bcl-2/Bax提高,超短波组与旋磁组间差异无统计学意义(P>0.05);在减少脑含水量和减小脑梗死体积方面,旋磁组与对照组比较,差异无统计学意义(P>0.05)。结论脑缺血再灌注后18h,应用超短波治疗对局灶性脑缺血再灌注损伤有显著疗效;本实验条件下旋磁治疗对改善脑水肿、减小脑梗死体积未见显著疗效,在改善凋亡方面有显著疗效。
Objective To investigate the effects of ultrashort wave and gyromagnetic treatment on focal cerebral ischemia-reperfusion injury in rats. Methods Focal cerebral ischemia-reperfusion model was established by thread occlusion method. Fifty - two healthy Wistar rats were randomly divided into sham operation group, control group, ultrashort wave treatment group (ultrashort wave group) and gyromagnetic treatment group (gyromagnetic group). After 18h of cerebral ischemia and reperfusion, the rats were given the treatment and the brains were harvested at 24 hours. The water content of brain tissue, the volume of cerebral infarction and the expression of Bcl-2 and Bax protein in brain tissue of each group were observed. Results Ultra-shortwave treatment reduced cerebral water content and infarction volume 18h after cerebral ischemia-reperfusion. The expressions of Bcl-2, Bax and Bcl-2 / Bax in ultrashort wave group and cyclophosphamide group increased, (P> 0.05). There was no significant difference in the volume of cerebral water and the volume of cerebral infarction when compared with the control group (P> 0.05). Conclusion 18h after cerebral ischemia-reperfusion, application of ultrashort wave therapy has a significant effect on focal cerebral ischemia-reperfusion injury; under the experimental conditions, gyromagnetic treatment has no significant effect on improving cerebral edema and reducing cerebral infarction volume, Apoptosis has a significant effect.