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通过水稻(Oryza sativa ssp.japonica)(品种通育211)与柳叶菜科植物月见草(Oenothera biennis)进行远缘杂交,在D2代出现变异株,D3～D6代出现了大量、明显的性状变异,并且出现特种米如巨胚米。对D6部分株系进行统计分析,在株高(变幅49.2～164.5cm)、每株穗数(12.1～38.2个)、穗长(20.3～30.3cm)、剑叶长(13.8～57.5cm)、叶宽(11.1～25.2mm)、主穗粒数(142.0～367.0粒)、结实率(0.8%～99.0%)、千粒重(19.7～33.8g)、生育期(90.0～108.0d)等性状与受体相比均达到极显著水平,变异系数多数在20%以上,最高达到40.8%。通过系谱选育,获得了一批在形态、生育期和多个农艺性状上有育种价值的水稻新种质,并出现了产量构成因素高度协调的材料,如DC621,在产量方面表现突出的材料约占30%～40%。对代表性变异材料进行了AFLP分子标记分析表明,月见草的花粉介入确实使受体水稻在DNA分子水平产生了大量的变异,DNA片段的丢失与增加普遍存在。
Distant hybridization was carried out between Oryza sativa ssp. Japonica (Oryza sativa ssp. Japonica) and Oenothera biennis (Oketo), and a mutant appeared in D2 generation. A large number of obvious Traits variation, and the emergence of special rice, such as giant embryo rice. According to the statistic analysis of some lines of D6, the variation of plant height (range 49.2-164.5cm), panicle number per plant (12.1-38.2), spike length (20.3-30.3cm) and flag leaf length (13.8-57.5cm) , Leaf width (11.1 ~ 25.2mm), main ear number (142.0 ~ 367.0), seed setting rate (0.8% ~ 99.0%), 1000-grain weight (19.7 ~ 33.8g), growth period (90.0 ~ 108.0d) Recipients reached extremely significant levels, the coefficient of variation in the majority of more than 20%, up to 40.8%. Through pedigree breeding, a number of new rice germplasms with breeding value in morphology, growth period and multiple agronomic traits were obtained, and materials with high coordination of yield components appeared, such as DC621, which showed outstanding performance in yield About 30% to 40%. AFLP molecular marker analysis of representative mutants showed that pollen intervention in Primula japonica indeed produced a large number of variation in the molecular level of DNA in recipient rice, and the loss and increase of DNA fragments were ubiquitous.