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目的研究海绵ｐｏｌｙｍｉｓｔｉａｓｐｏｎｇｉａ提取物ａｌｄｉｓｉｎ的抗脂质过氧化及清除自由基作用。方法采用ＭＤＡ－ＴＢＡ比色法测定组织匀浆中过氧化脂质（ＬＰＯ）含量；采用邻苯三酚自氧化法测定ａｌｄｉｓｉｎ对Ｏ·２自由基的清除作用；采用分光光度法测定ａｌｄｉｓｉｎ对Ｃｕ２＋－ＶｉｔＣ体系产生的·ＯＨ自由基的清除作用；采用ＥＳＲ法测定ａｌｄｉｓｉｎ对ＤＰＰＨ自由基的清除作用。结果ａｌｄｉｓｉｎ具有显著的抗脂质过氧化作用，并能有效地清除Ｏ·２、·ＯＨ和ＤＰＰＨ自由基，在０．４２６μｍｏｌ·Ｌ－１～１８．５ｍｍｏｌ·Ｌ－１浓度范围内有明显的量效关系。结论ａｌｄｉｓｉｎ是自由基清除剂。
Objective To study the anti-lipid peroxidation and free radical scavenging effects of sponge polymistiaspongia extract aldisin. Methods The content of lipid peroxidation (LPO) in tissue homogenate was determined by MDA-TBA colorimetric assay. The scavenging effect of aldisin on O · 2 free radical was measured by pyrogallol autoxidation method. The effect of aldisin on Cu2 + -VitC system · OH radical scavenging effect; ESR determination of aldisin DPPH free radical scavenging effect. Results aldisin had significant anti-lipid peroxidation and was able to effectively scavenge O · 2, · OH and DPPH free radicals. In the concentration range of 0.426μmol·L-1 ~ 18.5mmol·L-1, aldisin had significant Quantitative effect relationship. Conclusion aldisin is a free radical scavenger.