采用胚胎肢芽细胞进行原代微团培养 ,培养物经不同浓度的五氧化二钒 (V2 O5 )对细胞肢芽细胞经 5天增减形成细胞集落 ,V2 O5 对体外肢芽细胞的分化有明显抑制作用 ,并有较明显的剂量 -效应关系 ,其半数生长抑制浓度(ICV5 0 )和半分化抑制浓度 (ICD5 0 )分别为 12 .6和 1.6μg ml,V D值为 7.9。表明抑制细胞分化可能是V2 O5 致发育危害的重要作用机理 Primary limbular bud cells were used to culture primary micelles. Cultured cells were treated with different concentrations of vanadium pentoxide (V2 O5) for 5 days to form cell colonies. The differentiation of V2 O5 to limb bud cells (ICV50) and IC50 (ICD50) were 12.6 and 1.6μg ml, respectively. The VD value was 7.9. Indicating that inhibition of cell differentiation may be V2 O5 caused by the development of an important mechanism of action