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目的:探讨LPXN过表达对白血病细胞株THP-1增殖、粘附和侵袭的影响及其可能机制。方法:构建稳定过表达LPXN的THP-1细胞。CCK-8检测细胞增殖能力;粘附实验检测细胞对Fn的粘附能力;Transwell实验检测细胞侵袭能力;Western blot检测ERK,Integrinα4,α5和β1表达;明胶酶谱法检测细胞分泌MMP2和MMP9活性的变化。结果:成功构建稳定过表达LPXN的THP-1细胞。过表达LPXN的THP-1细胞增殖、对Fn的粘附、ERK,Integrinα4,α5和β1表达均高于对照组。过表达LPXN的THP-1细胞跨transwell的侵袭能力和MMP2活性均强于对照组。结论:LPXN基因可能通过上调ERK的表达促进THP-1细胞增殖;通过上调Integrinα4、α5、β1的表达促进THP-1细胞的粘附;通过激活M M P2促进THP-1细胞侵袭。
Objective: To investigate the effect of LPXN overexpression on proliferation, adhesion and invasion of leukemia cell line THP-1 and its possible mechanism. Methods: THP-1 cells stably overexpressing LPXN were constructed. CCK-8 was used to detect the proliferation of cells; Adhesion assay was used to detect cell adhesion to Fn; Transwell assay was used to detect cell invasion; Western blot was used to detect the expression of ERK, Integrinα4, α5 and β1; Gelatin zymography The change. RESULTS: THP-1 cells stably over-expressing LPXN were successfully constructed. Proliferation of THP-1 cells over-expressing LPXN, adhesion to Fn, ERK, Integrin α4, α5 and β1 expression were higher than the control group. THP-1 cells overexpressing LPXN had stronger transmembrane invasiveness and MMP2 activity than the control group. CONCLUSION: LPXN gene may promote the proliferation of THP-1 cells by increasing the expression of ERK. It may promote the adhesion of THP-1 cells by up-regulating the expression of Integrinα4, α5 and β1 and promote the invasion of THP-1 cells by activating M M P2.