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简要阐述了基因打靶的概念和基本环节 ,系统介绍了筛选方法和基因打靶的策略。基因打靶在基因功能研究、人类疾病动物模型的建立和基因治疗等方面有着广阔的应用前景
The concept and basic steps of gene targeting are briefly introduced. The screening methods and gene targeting strategies are introduced systematically. Gene targeting has broad application prospects in the research of gene function, animal model of human disease and gene therapy