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目的探索Toll样受体2(Toll-likereceptor2,TLR2)在小鼠肝脏部分缺血再灌注过程中在缺血肝叶中的激活,分析TLR2的激活与肝功能损伤之间的关系。方法BALB/c小鼠复制肝脏部分缺血再灌注(缺血1h再灌注4h)损伤动物模型(I/R组),假手术对照组(SH组)同样行开腹手术但不夹闭血管。采用实时荧光定量PCR(Real-time-PCR)方法定量检测缺血肝叶中TLR2mRNA的表达变化,同时采用Westernblot检测缺血肝脏组织中TLR2蛋白的表达变化,检测门静脉血浆丙氨酸氨基转移酶(pALT)水平。结果肝脏部分缺血1h再灌注4h后,I/R组与SH组小鼠缺血肝叶TLR2mRNA的表达(ΔCt值)分别为(1.06±0.91)vs(5.08±1.32)(P<0.01),由于ΔCt值越小则基因表达水平越高,说明缺血再灌注过程中缺血肝叶TLR2mRNA的表达水平增高。且I/R组小鼠缺血肝叶TLR2蛋白的表达(OD值)水平较SH组高[(433.91±25.53)vs(102.86±13.58),P<0.01],I/R组中门静脉血清ALT水平升高[(848.33±271.37)μ/Lvs(42.39±14.75)μ/L,P<0.01]。结论TLR2在肝脏缺血再灌注过程中缺血肝叶的表达增强,且这种变化与肝功能的损伤相关。
Objective To explore the activation of Toll-like receptor 2 (TLR2) in ischemic liver lobe during partial hepatic ischemia-reperfusion in mice and analyze the relationship between TLR2 activation and hepatic injury. Methods BALB / c mice were sacrificed to establish an animal model of ischemia / reperfusion (reperfusion 4h after ischemia). The sham operation group (SH group) underwent laparotomy without clamping the blood vessels. The real-time quantitative PCR (Real-time-PCR) was used to quantitatively detect the expression of TLR2 mRNA in ischemic liver lobe. Meanwhile, the expression of TLR2 protein in ischemic liver tissue was detected by Western blot. The level of alanine aminotransferase pALT) levels. Results The expression of TLR2mRNA in ischemic liver livers of I / R group and SH group was (1.06 ± 0.91) vs (5.08 ± 1.32) (P <0.01) after partial ischemia for 1h and reperfusion for 4h respectively. The lower the ΔCt value, the higher the gene expression level, indicating that the expression of TLR2 mRNA in ischemic liver lobe during ischemia-reperfusion increased. (433.91 ± 25.53) vs (102.86 ± 13.58), P <0.01]. The serum level of ALT in portal vein in I / R group was higher than that in SH group The level of [(848.33 ± 271.37) μ / Lvs (42.39 ± 14.75) μ / L, P <0.01]. Conclusion The expression of TLR2 in hepatic ischemia-reperfusion liver livers is enhanced, and this change is related to the damage of liver function.