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目的研究慢性丙型肝炎患者肝免疫标志物和病毒基因型的关系。方法取28例HFE基因型患者的肝活检标本进行切片染色,并通过形态测定法分析CD8,主要组织相容性复合体-Ⅰ类(majorhistocompatibilitycomplexclassⅠ,MHC-Ⅰ),β2微球蛋白(β2microglobulin,β2-mG),HFE和CD68。其中18例患者的治疗应答性资料有效。结果CD8+常聚集于肝汇管区和窦状隙,CD8+与MHC-Ⅰ阳性内样细胞相互作用。MHC-Ⅰ和β2-mG主要表达于内皮细胞和枯否细胞,而大多数HFE表达于圆形和树突状CD68+细胞。基因型为3a的丙型肝炎患者肝MHC-Ⅰ和HFE强表达,且对干扰素治疗持续应答率较高。结论慢性丙型肝炎患者MHC-Ⅰ的肝脏表达可能与病毒基因型相关。HCV3a基因型上调MHC-Ⅰ和HFE的表达。
Objective To study the relationship between liver immune markers and viral genotypes in patients with chronic hepatitis C Methods Totally 28 liver biopsy specimens from patients with HFE genotype were stained by immunohistochemistry. The expression of CD8, major histocompatibility complex class I (MHC-Ⅰ), β2 microglobulin (β2 -mG), HFE and CD68. Among 18 patients, the response data were valid. Results CD8 + often clustered in the hepatic portal area and sinusoids, and CD8 + interacted with MHC-I positive endogenous cells. MHC-I and β2-mG are mainly expressed in endothelial cells and Kupffer cells, whereas most HFEs are expressed in round and dendritic CD68 + cells. Hepatitis C patients with genotype 3a had a strong expression of MHC-I and HFE, and had a sustained response rate to interferon therapy. Conclusion The liver expression of MHC-Ⅰ in patients with chronic hepatitis C may be related to the virus genotype. HCV3a genotype up-regulates MHC-I and HFE expression.