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将本室合成、克隆的马铃薯卷叶病毒（ＰｏｔａｔｏＬｅａｆｒｏｌＶｉｒｕｓ，ＰＬＲＶ）中国分离株的基因间隔区（ｉｎｔｅｒｇｅｎｉｃｓｅｑｕｅｎｃｅ，ＩＳ）双链ｃＤＮＡ以正、反向两种方式分别构建于转化载体ｐＲＯＫ２中，通过致瘤农杆菌介导，以马铃薯叶圆片为转化材料，转化马铃薯栽培品种Ｄｅｓｉｒｅ，获得了转基因植株。卡那霉素抗性分析和ＰＣＲ检测目的基因，证明ＰＬＲＶＩＳ双链ｃＤＮＡ已经整合到转基因马铃薯的染色体基因组中。将转基因植株移栽网棚用蚜虫接种ＰＬＲＶ，观察症状并用酶联免疫吸附测定（ＥＬＩＳＡ）检测转基因植株中ＰＬＲＶ含量。结果表明，表达ＰＬＲＶＩＳ正意和反意ＲＮＡ的转基因植株，接种病毒后表现无症状或症状轻微，ＰＬＲＶ平均滴度均较未转基因对照植株低。表达正意ＲＮＡ的转基因植株ＰＬＲＶ滴度降低４３％～７２％，表达反意ＲＮＡ的转基因植株ＰＬＲＶ滴度降低７２％～８６％，由此可见，表达ＰＬＲＶＩＳ反意ＲＮＡ的转基因马铃薯对ＰＬＲＶ抗性较强。
The intergenic sequence (IS) double-stranded cDNA of PotatoLeafrolVirus (PLRV) isolated from China was cloned into the transformation vector pROK2 in both positive and negative directions. Agrobacterium tumefaciens-mediated transformation of potato leaf discs as a transformation material, transformation of potato cultivar Desire, obtained transgenic plants. Kanamycin resistance analysis and PCR detection of the target gene, proved that PLRVIS double-stranded cDNA has been integrated into the genome of transgenic potato genome. PLRV was inoculated with aphids in the cymbal of transplanting plants and the symptoms were observed. PLRV content in the transgenic plants was detected by enzyme-linked immunosorbent assay (ELISA). The results showed that transgenic plants expressing both positive and negative PLRVIS RNA showed no symptom or slight symptom after inoculation with virus, and the average PLRV titer was lower than that of non-transgenic control plants. PLRV titers of transgenic plants expressing positive RNA decreased by 43% -72%, and PLRV titers of transgenic plants expressing anti-sense RNA decreased by 72% -86%. Thus, transgenic potato expressing PLRVIS anti-sense RNA showed no resistance to PLRV Strong.