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本文采用受体放射自显影技术，对氟哌啶加强针刺镇痛时脑内阿片受体结合位点的变化进行观察，受体的标记配基为３Ｈ－依托芬。结果表明，针刺镇痛时大鼠脑内３Ｈ－依托芬的结合位点在许多核团明显增加，如中脑导水管周围灰质、尾核、伏核、视前区、海马、缰核、丘脑、下丘脑、上丘、脚间核、黑质、杏仁核、脊髓背角等，当氟哌啶与针刺合用并加强了针刺镇痛时，上述核团内阿片受体的结合位点进一步增加。本文结果提示，内阿片受体的激活是实现针刺镇痛的条件之一，内阿片受体功能的进一步增强，可能是氟哌啶加强针刺镇痛的机制之一。Ｐ＜０．０５．ｖｓＮＳｇｒｏｕｐ；＋Ｐ＜０．０５，＋＋ｐ＜０．ｏｌ，ｖｓＮＳ＋ＥＡｇｒｏｕｐ；＊Ｐ＜０．０５，＂ｕｐ＜０．ｏｌ．ｖｓＤｇｒｏｕｐ￣ＳｅＯｆＭ￣ｄｅｒｐｔｈｅｂ￣２０ｒａｔＳｗｅｒｅｄｉｖｉｄｅｄｉｎｔｏｆｏｕｒｇｒｏｕｐｓａｓｍｅｎｈｏｎｅｄａｂｏｖｅ．ＴｈｅａｎｉｍａｌｓｗｅｒｅＳａｃｒｉｆｉｃｅｄｂｙｄｍｐｉｔａｈｏｎａｔｔｈｅ￣ｍｕｍｏｆｐａｉｎ￣ｏｌｄａｐｐｅａｌｓ（ａｂｏｕｔ２０ＩｎｉｎｆｏｌｌｏｗｉｎｇＥＡ）．Ｐａｉｎｔｏｅｈｏｌｄｗａｓｍｅａ￣ｕｎｔｉｌｔｈｅｄａｓｐｉｔａｈｏｎ．Ａｎｔｏｒａｄ
In this paper, receptor autoradiography was used to observe the change of intracerebral opioid receptor binding site of haloperidol in acupuncture and analgesia. The labeled ligand of the receptor was 3H-etophor. The results showed that the binding sites of 3H-ethenfentine in rat brain increased obviously in many nuclei during acupuncture analgesia, such as gray matter, caudate nucleus, nucleus accumbens, presynaptic zone, hippocampus, habenula, The thalamus, hypothalamus, superior colliculus, intermediate nucleus, substantia nigra, amygdala, spinal dorsal horn, etc. When haloperidol is combined with acupuncture and acupuncture analgesia is strengthened, the binding sites Point to further increase. Our results suggest that the activation of endopioid receptor is one of the conditions to achieve acupuncture analgesia. The further enhancement of endopioid receptor function may be one of the mechanisms by which droperidol can enhance acupuncture analgesia. P <0.05. vsNSgroup; + P <0.05, ++ p <0. ol vsvs + EAgroup; * P <0.05, "up <0.ol.vsDgroup ~ SeOfM ~ derptheb ~ 20ratSweredividedintofourgroupsmenhonedabove.TheanimalswereSacrificedbydmpitahonatthe ~ mofofpain¯oldappeals (about20InfollowingEA) .Paintoeholdwasmea ~ untilthedaspitahon.Antorad