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目的 探讨１ 型糖尿病胰岛素缺乏机理及 Ｇ Ａ Ｄ Ａｂ 对胰岛细胞结构及功能的影响。方法 以改良的放射配体法筛选１ 型糖尿病患者 Ｇ Ａ Ｄ Ａｂ 阳性血清，以亲和层析法从该血清中分离纯化 Ｇ Ａ Ｄ Ａｂ ，以免疫荧光法观察 Ｇ Ａ Ｄ Ａｂ 与β细胞的结合；将纯化的 Ｇ Ａ Ｄ Ａｂ 及／ 或补体加入人胎胰岛细胞培养体系。结果 Ｇ Ａ Ｄ Ａｂ 能够与β细胞结合，并使培养胰岛细胞的胰岛素释放量减少；透射电镜观察，加 Ｇ Ａ Ｄ Ａｂ 组β细胞胞浆颗粒减少；同时加入补体组β细胞的细胞膜被破坏。结论 １ 型糖尿病患者血清 Ｇ Ａ Ｄ Ａｂ 在体外能够抑制培养的人胎胰岛β细胞的胰岛素合成和释放，并且有补体依赖性细胞毒作用。
Objective To investigate the mechanism of insulin deficiency in type 1 diabetes and the effect of G A D Ab on the structure and function of islet cells. Methods G A DAb positive serum was screened by modified radioligand method in patients with type 1 diabetes mellitus, and G A D Ab was purified from the serum by affinity chromatography. β-cell binding; the purified G A D Ab and / or complement into human fetal islet cell culture system. Results G A D Ab with β-cell binding and insulin secretion of cultured islets decreased; transmission electron microscopy, plus G A D Ab group decreased β-cell cytoplasmic granules; while adding complement group β cells The cell membrane is destroyed. Conclusion Serum G A D Ab in patients with type 1 diabetes can inhibit the insulin synthesis and release in cultured human fetal pancreatic β-cells in vitro and has a complement-dependent cytotoxicity.