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目的探讨糖尿病大鼠肾脏中Toll样受体4(TLR4)的表达及与巨噬细胞浸润的关系。方法 STZ注射制备糖尿病大鼠模型。用免疫组织化学法检测大鼠肾脏巨噬细胞的浸润及TLR4的表达。用Western blot及RT-PCR方法检测大鼠肾脏TLR4蛋白及mRNA的表达。结果成模后,TLR4在肾小管的表达增加。DM组大鼠2、4、8周TLR4/β-actin表达高于NC组。DM组2、4、8周TLR4/GAPDH mRNA表达均高于NC组。DM组4、8周肾间质巨噬细胞浸润数均高于NC组。结论在糖尿病大鼠肾脏中,TLR4表达上调,与炎症浸润相关。
Objective To investigate the relationship between the expression of Toll-like receptor 4 (TLR4) and macrophage infiltration in the kidney of diabetic rats. Methods STZ injection of diabetic rat model. Immunohistochemistry was used to detect the infiltration of macrophages in rat kidney and TLR4 expression. Western blot and RT-PCR were used to detect the expression of TLR4 protein and mRNA in rat kidney. Results After modeling, the expression of TLR4 in the renal tubules increased. The expression of TLR4 / β-actin in DM group was higher than that in NC group at 2,4,8 weeks. The mRNA expression of TLR4 / GAPDH in DM group was higher than that in NC group at 2, 4 and 8 weeks. The number of renal interstitial macrophages in DM group was higher than that in NC group at 4 and 8 weeks. Conclusion In the kidney of diabetic rats, the expression of TLR4 is up-regulated, which is correlated with inflammatory infiltration.