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目的:探讨十溴联苯醚(BDE-209)对人卵巢癌细胞株OVCAR-3及中国仓鼠卵巢上皮细胞株CHO细胞增殖和细胞周期的影响。方法:采用MTT检测法、免疫荧光化学检测法以及流式细胞仪等方法观察不同浓度BDE-209(0～100nmol/L)作用于OVCAR-3和CHO细胞0～72h,其细胞形态学变化、OD值改变、K i67表达变化及细胞周期的变化。结果:(1)0～100nmol/L BDE-209作用于细胞72h,倒置显微镜下观察随着浓度增高细胞增殖明显,细胞间隙变小甚至消失,细胞密集排布,细胞形态变小;(2)MTT法检测发现BDE-209促进两种细胞增殖,其增殖率呈时间依赖性和浓度依赖性;(3)0～100nmol/L BDE-209作用72h后,K i67阳性细胞数量增多,表达强度增强,呈现浓度依赖性;(4)流式细胞术检测发现BDE-209改变了细胞周期分布,OVCAR-3细胞G2/M期及CHO细胞S期细胞比例明显增高,且增殖指数(PI)随药物浓度增加而升高。结论:BDE-209明显促进OVCAR-3细胞和CHO细胞体外增殖,并使G2/M期或S期细胞比例增加。
Objective: To investigate the effects of decabromodiphenyl ether (BDE-209) on the proliferation and cell cycle of human ovarian cancer cell line OVCAR-3 and Chinese hamster ovary epithelial cell line CHO cells. Methods: MTT assay, immunofluorescence assay and flow cytometry were used to observe the morphological changes of OVCAR-3 and CHO cells exposed to different concentrations of BDE-209 (0-100nmol / L) for 0-72h. OD value changes, K i67 expression changes and cell cycle changes. The results showed that: (1) BDE-209 treated with 0-100nmol / L BDE-209 for 72h, under inverted microscope, the cell proliferation was obvious with the concentration increasing, the cell gap became smaller or even disappeared, the cells were densely arranged, MTT assay showed that BDE-209 promoted the proliferation of both cell lines in a time-dependent and concentration-dependent manner. (3) After 72h with 0-100nmol / L BDE-209, the number of K i67 positive cells increased and the expression intensity increased (4) The results of flow cytometry showed that BDE-209 changed the cell cycle distribution, the proportion of G2 / M phase in OVCAR-3 cells and S phase in CHO cells was significantly increased, and the proliferation index (PI) Concentration increased and increased. CONCLUSION: BDE-209 can significantly promote the proliferation of OVCAR-3 and CHO cells in vitro and increase the proportion of cells in G2 / M phase or S phase.