在花药培养过程中,由于某个环节灭菌不彻底,常造成接种后的培养基污染,如不设法去除,污染点会逐步扩大,最终使材料报废。为排除培养瓶中的污染点,一般采用匙挖去污法。此法需挖几次才能去除;对已形成孢子体的菌落,挖除时的震动,又会使孢子散落,造成再污染。 In the process of anther culture, due to a certain part of the sterilization is not complete, often resulting in media inoculation after the pollution, if not managed to remove the pollution point will gradually expand and eventually scrapped the material. In order to rule out the pollution point in the culture bottle, generally adopt key dig decontamination method. This method need to dig several times to remove; have formed spores of colonies, digging when the vibration, will make spores scattered, resulting in re-contamination.