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本文建立了特异性测定全血中环孢素Ａ（ＣｓＡ）的高效液相法（ＨＰＬＣ），并与荧光偏振免疫法（ＦＰＩＡ）比较，结果提示两法相关性好，回归方程ＦＰＩＡ值＝７５．６８＋２．６８ＸＨＰＬＣ值（Ｎ＝９４），ｒ＝０．９０５（Ｐ＜０．００１），ＨＰＬＣ法测定的是ＣｓＡ主药和代谢物，ＦＰＩＡ／ＨＰＬＣ值＞３．提示患者体内ＣｓＡ代谢物积蓄，肝功能异常。
A HPLC method was developed for the determination of cyclosporin A (CsA) in whole blood. Compared with fluorescent polarization immunoassay (FPIA), the results showed that the correlation between the two methods was good and the FPIA value of regression equation was 75. 68 + 2.68XHPLC value (N = 94), r = 0.905 (P <0.001). The main drug and metabolite of CsA were determined by HPLC with FPIA / HPLC> 3. Tip CsA metabolites in patients with accumulation, abnormal liver function.