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目的:构建乳腺癌体外骨转移瘤3D模型。方法:新生CD-1小鼠的颅骨单独孵育为正常组,新生CD-1小鼠的颅骨与MDA-MB-231细胞低氧共孵育四天为模型组。通过扫描电镜(SEM)鉴定骨转移瘤模型,中性红染色法鉴定骨组织中的破骨细胞,硝酸银复染法观察骨的溶解,结晶紫染色法观察骨转移瘤模型中肿瘤细胞的生长。结果:正常组骨表面光滑完整;模型组骨组织表面黏附大量肿瘤细胞,破骨细胞活性增强,产生严重的溶骨,表面出现骨陷窝,骨纤维发生断裂。结论:成功建立了乳腺癌骨转移瘤体外3D模型,该模型能够模拟体内骨转移瘤微环境。
Objective: To construct a 3D model of breast cancer in vitro bone metastases. Methods: The skull of neonatal CD-1 mice were individually incubated with normal group. The skull of neonatal CD-1 mice were co-incubated with hypoxia of MDA-MB-231 cells for 4 days. The model of bone metastases was identified by scanning electron microscopy (SEM). The osteoclasts in bone were identified by neutral red staining. The bone was lysed by silver nitrate staining. The growth of tumor cells in bone metastasis model was observed by crystal violet staining . Results: The bone surface of the normal group was smooth and intact. In the model group, a large number of tumor cells were adhered on the surface of the bone tissue, the activity of osteoclasts was enhanced, severe osteolytic bone was formed, and the lacunar fossa was found on the surface. Conclusion: The in vitro 3D model of bone metastasis of breast cancer was established successfully. This model can simulate the microenvironment of bone metastases in vivo.