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破伤风类毒素的浓缩与精制法,可分两大类。第一、化学方法,即用蛋白质沉淀剂将类毒素沉淀,然後将分离的类毒素溶解於缓冲液内。第二、物理方法,即用吸收剂将有效物质吸收,然後移去类毒素;或用活性碳吸收杂质或用超滤法。化学方法大约可分为:(1)中性盐沉淀与分提法:如用硫酸铵为沉淀剂。(2)重金属沉淀法:如用硫酸镉,氯化锌,磷酸钙为沉淀剂。
Tetanus toxoid concentration and refining method can be divided into two categories. First, a chemical method, that is, precipitation of the toxoid with a protein precipitating agent, and then dissolving the isolated toxoid in a buffer. Second, the physical method, which absorbs the active substance with an absorbent and then removes the toxoid, or absorbs impurities with activated carbon or ultrafiltration. Chemical methods can be divided into: (1) neutral salt precipitation and fractionation method: such as ammonium sulfate precipitant. (2) heavy metal precipitation method: such as cadmium sulfate, zinc chloride, calcium phosphate as a precipitating agent.