论文部分内容阅读
目的:建立利用自微乳浸提串联浊点浓缩分离蛇床子中香豆素类化合物的工艺。方法:以蛇床子素溶解量为指标,通过单因素试验筛选油相、乳化剂、助乳化剂,利用伪三元相图优化自微乳处方;以蛇床子素和总香豆素提取量为指标,采用U5(54)均匀试验考察浸提温度、时间、液固比和超声功率对提取工艺的影响,通过浊点浓缩分离自微乳提取液中香豆素类成份。结果:优选的微乳液处方为[聚乙二醇辛基苯基醚-正丁醇(5∶1)]-油酸乙酯-水(2.5∶0.5∶7);最优工艺参数为液固比20∶1,提取温度50℃,超声功率280 W,浸提时间60 min,蛇床子素和总香豆素的提取率分别达83.2%,91.19%;利用浊点浓缩方法处理自微乳提取液,油层中蛇床子素和总香豆素的回收率分别达90.33%,78.91%。结论:该工艺简单易行,适用于蛇床子活性成分的提取和浓缩分离。
OBJECTIVE: To establish a process for the separation and separation of coumarins from Cnidium monnieri by self-microemulsification and tandem cloud point. Methods: Taking the ostholeololysis as the index, the oil phase, the emulsifier and the co-emulsifier were screened through the single factor test. The pseudo-ternary phase diagram was used to optimize the self-microemulsion prescription. The osthol and total coumarin Indicators, using U5 (54) uniform test study extraction temperature, time, liquid-solid ratio and ultrasonic power on the extraction process, separated by cloud point concentration from the microemulsion extract coumarin components. Results: The preferred microemulsion formulation was [polyethylene glycol octylphenyl ether n-butanol (5: 1)] -oleic acid ethyl ester water (2.5: 0.5: 7) Extraction ratio of 20: 1, extraction temperature of 50 ℃, ultrasonic power of 280 W and extraction time of 60 min, respectively. The extraction rates of Osthole and total coumarin were 83.2% and 91.19% respectively. The recoveries of osthol and total coumarin in liquid and oil layers were 90.33% and 78.91% respectively. Conclusion: The process is simple and easy to apply for the extraction and concentration separation of the active components of Cnidium.